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作物学报 ›› 2017, Vol. 43 ›› Issue (07): 1012-1020.doi: 10.3724/SP.J.1006.2017.01012

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

茶树CsbZIP4转录因子正调控拟南芥对盐胁迫响应

曹红利1,2,**,王璐1,**,钱文俊1,郝心愿1,杨亚军1,*,王新超1,*   

  1. 1中国农业科学院茶叶研究所 / 国家茶树改良中心 / 农业部茶树生物学与资源利用重点实验室, 浙江杭州310008; 2福建农林大学园艺学院, 福建福州350002
  • 收稿日期:2016-10-21 修回日期:2017-03-01 出版日期:2017-07-12 网络出版日期:2017-03-24
  • 通讯作者: 王新超, E-mail: xcw75@tricaas.com, Tel: 0571-86653162; 杨亚军, E-mail: yjyang@tricaas.com.
  • 基金资助:

    本研究由浙江省自然科学基金(LY14C160001), 国家自然科学基金项目(31500564), 国家现代农业产业技术体系建设专项(CARS-23)和中国农业科学院农业科技创新工程项目(CAAS-ASTIP-2014-TRICAAS)资助。

Positive Regulation of CsbZIP4 Transcription Factor on Salt Stress Response in Transgenic Arabidopsis

CAO Hong-Li1,2,**,WANG Lu1,**,QIAN Wen-Jun1,HAO Xin-Yuan1,YANG Ya-Jun1,*,WANG Xin-Chao1,*   

  1. 1Tea Research Institute, Chinese Academy of Agricultural Sciences / National Center for Tea Improvement / Key Laboratory of Tea Biology and Resources Utilization, Ministry of Agriculture, Hangzhou 310008, China; 2College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002, China
  • Received:2016-10-21 Revised:2017-03-01 Published:2017-07-12 Published online:2017-03-24
  • Contact: Wang Xinchao, E-mail: xcw75@tricaas.com, Tel: 0571-86653162;Yang Yajun, E-mail: yjyang@tricaas.com.
  • Supported by:

    This study was supported by the Natural Science Foundation of Zhejiang Province (LY14C160001), the National Natural Science Foundation of China (31500564), the Earmarked Fund for China Agriculture Research System (CARS-23), and the Chinese Academy of Agricultural Sciences through an Innovation Project for Agricultural Sciences and Technology (CAAS-ASTIP-2014-TRICAAS).

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摘要：

bZIP转录因子是真核生物中一类多功能蛋白家族, 参与种子成熟、光信号调节、胁迫响应等多种生物学过程, 拟南芥中根据序列相似性和保守域主要分为10个亚家族(A-I和S)。本文以茶树的C亚家族转录因子CsbZIP4为研究对象, 调查非生物胁迫下的表达模式, 及转化拟南芥后CsbZIP4过表达对耐盐性的影响。结果显示, 在4℃低温、外源ABA、盐和干旱胁迫处理后, CsbZIP4的表达在茶树叶片中呈上调模式, 特别是在盐和干旱胁迫下其表达分别上调2.9倍和2.2倍; 而在根中, 低温、盐和干旱胁迫均能显著抑制CsbZIP4的表达, 其中盐胁迫能将其表达抑制2倍; 荧光显微镜下观察CsbZIP4-GFP融合蛋白, 将CsbZIP4定位于细胞核中; CsbZIP4的过表达能够降低转基因株系种子萌发时对外源ABA、盐胁迫的敏感性, 在300 mmol L–1 NaCl盐胁迫下, 转化拟南芥植株过表达CsbZIP4增强抗性, 其叶片的SPAD值较高, 同时过表达株系中盐胁迫响应基因AtSOS1的表达显著增强。根据CsbZIP4正调控拟南芥的盐胁迫响应, 推断CsbZIP4与茶树抵御盐胁迫密切相关。

关键词: 茶树, CsbZIP4转录因子, 非生物胁迫, 耐盐性

Abstract:

Basic region / leucine zipper (bZIP) transcription factors is a multi-functional protein family in eukaryotes, which is involved in various biological processes including seed maturation, light signaling regulation and stress response. According to sequence similarity and conserved motifs, the AtbZIPs are classified into 10 groups (A, B, C, D, E, F, G, H, I, and S). In this study, CsbZIP4 of C-group bZIP transcription factors was as an objective, the expression patterns of CsbZIP4 in response to abiotic stress in tea plants were investigated, and the salinity tolerance caused by CsbZIP4 overexpression in Arabidopsis was analyzed. The CsbZIP4 from tea plant leaves was up-regulated under 4°C, exogenous ABA, salinity and dehydration stresses, especially induction of salinity and dehydration stresses led to up-regulation of 2.9-fold and 2.2-fold, respectively. In roots, CsbZIP4 was down-regulated in response to cold, salinity and dehydration stresses, especially down-regulated by 2-fold under salinity stress. Under fluorescence microscope, it was indicated that CsbZIP4 was located in nucleus. Constitutive overexpression of CsbZIP4 in transgenic Arabidopsis lowered the plants’ sensitivity to exogenous ABA and salinity at germination stage. Moreover, CsbZIP4 overexpression lines exhibited higher salinity tolerance under 300 mmol L–1 NaCl conditions and higher SPAD values. AtSOS1, which is a salinity responsive gene, was strongly induced in overexpression lines. In conclusion, CsbZIP4 positively regulates salt stress response in Arabidopsis, and might be closely related to the tolerance to salinity stress in tea plant.

Key words: Tea plant, CsbZIP4 transcription factor, Abiotic stress, Salinity tolerance

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