欢迎访问作物学报,今天是

作物学报 ›› 2022, Vol. 48 ›› Issue (3): 759-769.doi: 10.3724/SP.J.1006.2022.14025

• 研究简报 • 上一篇    下一篇

甘蓝型油菜白花基因InDel连锁标记开发

王瑞1,2(), 陈雪1,2, 郭青青1,2, 周蓉1,2, 陈蕾1,2, 李加纳1,2,*()   

  1. 1西南大学农学与生物科技学院, 重庆 400715
    2重庆市油菜工程技术研究中心, 重庆 400715
  • 收稿日期:2021-02-08 接受日期:2021-07-12 出版日期:2022-03-12 网络出版日期:2021-08-09
  • 通讯作者: 李加纳
  • 作者简介:E-mail: Ruiwang71@163.com
  • 基金资助:
    高等学校学科创新引智计划(111计划)项目(B12006)

Development of linkage InDel markers of the white petal gene based on whole-genome re-sequencing data in Brassica napus L.

WANG Rui1,2(), CHEN Xue1,2, GUO Qing-Qing1,2, ZHOU Rong1,2, CHEN Lei1,2, LI Jia-Na1,2,*()   

  1. 1College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
    2Chongqing Engineering Research Center for Rapeseed, Chongqing 400715, China
  • Received:2021-02-08 Accepted:2021-07-12 Published:2022-03-12 Published online:2021-08-09
  • Contact: LI Jia-Na
  • Supported by:
    Project of Intellectual Base for Discipline Innovation in Colleges and Universities (111 Program)(B12006)

摘要:

碱基插入/缺失(InDel)是基因组上广泛分布的遗传变异形式。但甘蓝型油菜白花基因InDel连锁标记还未见有关研究报道。本研究以甘蓝型油菜双单倍体(doubled haploid, DH)纯系黄花Y05和甘蓝型油菜纯系白花W01杂交构建F2群体。在F2群体中选取30株极端纯白花和30株极端纯黄花构建叶片DNA子代池, 对亲本和DNA子代池进行30×重测序。以法国甘蓝型油菜Darmor-bzh为参考序列, QTL-seq流程和PoPoolation2流程相互结合鉴定白花基因候选区间, 2种方法均将白花基因定位于法国甘蓝型油菜Darmor-bzh C03染色体52~54 Mb区间。利用IGV软件可视化白花基因候选区间插入缺失(InDel)变异位点, 依据候选区间序列信息设计InDel引物, 聚丙烯酰胺凝胶电泳筛选到8个与白花基因连锁共分离的InDel标记。上述研究为甘蓝型油菜白花基因精细定位和分子标记辅助选育以及白花基因功能标记开发奠定了研究基础和工作思路。

关键词: 甘蓝型油菜, 重测序, 白花基因, InDel标记

Abstract:

InDel is widely distributed across the genome and occurs in a high density and large numbers in a genome. To date, the researches about linkage InDel markers of the white petal gene in B. napus L are very less yet. In this study, we constructed the F2 mapping population from the cross between DH Y05 (yellow petal) and DH W01 (white petal). Two bulks with 30 yellow petal lines and 30 white petal lines of F2 population were constructed by mixing an equal amount of DNA. Then two bulks and parents were performed 30× whole-genome re-sequencing. Darmor-bzh as the reference genome was aligned to sequence data from the two bulks and parents. QTL-seq and PoPoolation2 workflow were applied to identify the candidate region of the white petal gene. A major candidate region was identified on chromosome C03 (52-54 Mb) of Darmor-bzh. The insertion-deletion (InDel) sites can be visualized in candidate interval by Integrative Genomics Viewer (IGV). Based on these Indel variations, we used Vector and Blast to design InDel primers. Eight InDel markers closely linked to the white petal gene were screened by Polyacrylamide gel electrophoresis (PAGE). In summary, these results provide a basis for fine mapping white petal gene and InDel molecular marker located on functional genes as well as molecular marker assisted selection breeding.

Key words: Brassica napus L., re-sequencing, white petal genes, InDel markers

图1

染色体上∆(SNP-index)分布 蓝点: ∆(SNP-index)位点; 红线: 利用滑动窗口数计算∆(SNP-index)变化趋势; 绿线: 显著性为95%的阈值; 橘线: 显著性为99%的阈值。"

图2

白花基因定位于C03 (Chr13)染色体上"

图3

IGV可视化候选区间InDel变异"

表1

InDel引物序列"

引物名称
Primer name
Chr. C03 InDel 正向引物序列
Forward sequence (5°-3°)
反向引物序列
Reverse sequence (5°-3°)
InDel-2 52375493-52375505 D10 GCTAAGCGTGTATCTTATCCT CGGGAATGGCGATCTAAAAA
InDel-3 52396484 I14 GAAAAACCATTGGAAATGCC TGGGAAGTTTGTTAAAGGGT
InDel-4 52348627-52348640 D12 GACAAGTCTTGGACAGCTAA CGAGAGGAATCCAAAGATGT
InDel-5 52377696-52377718 D20 TAGAGAGAGCTTAGACCTCC CGGCTATGAGTGATGTTCTT
InDel-6 52374279 I12 GGTTCTCTCTCTCTTCAGGTT TAATACTAATCGAAATTGTTGTCCC
InDel-8 52358540-52358551 D11 ATGCAACATCCAAAACACCT TTTTGCTCTTTTCGCGTTTG
InDel-9 52358775-52358788 D11 TCTGCGATCACGAATACCT CAAATCGAAAGGAAAGGAACA
InDel-10 52373701-52373721 D19 ACGTTAAAACAGCTTCACTTTC TCCGTGTCTGCCAAGTATC

图4

黄花和白花亲本及F2代单株电泳 1: 黄花亲本; 2: 白花亲本; 3~13: F2群体11个黄花单株; 14~24: F2群体11个白花单株。M: 20 bp梯度。"

[1] 王汉中. 以新需求为导向的油菜产业发展战略. 中国油料作物学报, 2018, 40:613-617.
Wang H Z. New-demand oriented oilseed rape industry developing strategy. Chin J Oil Crop Sci, 2018, 40:613-617 (in Chinese with English abstract).
[2] 阴长发, 官春云. 油菜花色研究进展. 作物研究, 2013, 27:403-408.
Yin C F, Guan C Y. A review of rapeseed flower color. Crop Res, 2013, 27:403-408 (in Chinese with English abstract).
[3] 刘后利. 油菜的遗传和育种. 上海: 上海科学技术出版社, 1985. pp 76-77.
Liu H L. Heredity and Breeding of Rape. Shanghai: Shanghai Scientific and Technical Publishers, 1985. pp 76-77(in Chinese)
[4] Chen B, Heneen W, Jonsson R. Brassica napus L Brassica napus L. Plant Breed, 1988, 100:147-149.
doi: 10.1111/pbr.1988.100.issue-2
[5] 戚存扣, 傅寿仲. 甘蓝型油菜白花性状的遗传. 中国油料作物学报, 1992, 1(3):60-62.
Qi C K, Fu S Z. Genetic studies of white petals in Brassica napus L. Chin J Oil Crop Sci, 1992, 1(3):60-62 (in Chinese with English abstract).
[6] Pearson O H. Brassica oleracea L Brassica oleracea L. Am Naturalist, 1929, 63:561-565.
doi: 10.1086/280291
[7] 张豹. 甘蓝型油菜导入系构建、重要农艺性状QTL分析和白花基因克隆. 华中农业大学博士学位论文, 湖北武汉, 2015.
Zhang B. Development of Chromosome Segment Substitution Lines for QTL Analysis of Important Agronomic Traits and Cloning the White-flowered Gene in Brassica napus L. PhD Dissertation of Huazhong Agricultural University, Wuhan, Hubei, China, 2015 (in Chinese with English abstract).
[8] 张洁夫, 浦惠明, 戚存扣, 傅寿仲. 甘蓝型油菜花色性状的遗传研究. 中国油料作物学报, 2000, 22(3):1-4.
Zhang J F, Pu H M, Qi C K, Fu S Z. Inheritance of flower color character in oilseed rape Brassica napus L. Chin J Oil Crop Sci, 2000, 22(3):1-4 (in Chinese with English abstract).
[9] 王翊, 景尚友, 吴刚, 任丽杰. 甘蓝型油菜白花性状在杂交油菜育种中的应用. 黑龙江农业科学, 2003, (6):13-14.
Wang Y, Jing S Y, Wu G, Ren L J. Application of the character of white flower of Brassica napus L. in hybrid breeding. Heilongjiang Agric Sci, 2003, (6):13-14 (in Chinese with English abstract).
[10] 文雁成, 张书芬, 王建平, 朱家成, 赵磊. 甘蓝型油菜白花性状的遗传学研究和白花胞质雄性不育系的选育. 中国农学通报, 2010, 26(1):95-97.
Wen Y C, Zhang S F, Wang J P, Zhu J C, Zhao L. Genetic studies of white Petals and selection of cytoplasmic male sterile line with white petals in Brassica napus L. Chin Agric Sci Bull, 2010, 26(1):95-97 (in Chinese with English abstract).
[11] 黄镇, 许婷, 班元元, 刘欢, 范胜栩, 杨丽, 徐爱遐. 甘蓝型油菜白花性状的遗传及AFLP标记. 华北农学报, 2012, 27(1):98-101.
Huang Z, Xu T, Ban Y Y, Liu H, Fan S X, Yang L, Xu A X. Genetic studies of white petals and AFLP markers linked to white petal gene in Brassica napus L. Acta Agric Boreali-Sin, 2012, 27(1):98-101 (in Chinese with English abstract).
[12] 邓昌蓉, 赵志刚, 余青兰. 人工合成甘蓝型油菜花色变异后代的遗传研究. 北方园艺, 2014, (18):14-17.
Deng C R, Zhao Z G, Yu Q L. The genetic studies of the flower color variation’s offsprings in artificial synthesis of Brassica napus. Northern Hortic, 2014, (18):14-17 (in Chinese with English abstract).
[13] 董育红, 田建华, 李殿荣, 郭蔼光, 孔建, 赵小萍. 甘蓝型油菜白花基因的RAPD标记. 西北农林科技大学学报, 2005, 33(10):57-61.
Dong Y H, Tian J H, Li D R, Guo A G, Kong J, Zhao X P. RAPD markers linked to white-petal gene in Brassica napus L. J Northwest A&F Univ, 2005, 33(10):57-61 (in Chinese with English abstract).
[14] 黄萌, 张建栋, 陈培峰, 宋英, 孙华. 甘蓝型油菜白花性状的遗传规律. 江苏农业科学, 2017, 45(20):83-84.
Huang M, Zhang J D, Chen P F, Song Y, Sun H. Genetic law of white flower traits in Brassica napus L. Jiangsu Agric Sci, 2017, 45(20):83-84 (in Chinese with English abstract).
[15] 田露申, 牛应泽, 余青青, 郭世星, 柳丽. 甘蓝型油菜白花性状的主基因+多基因遗传分析. 中国农业科学, 2009, 42:3987-3995.
Tian L S, Niu Y Z, Yu Q Q, Guo S X, Liu L. Genetic analysis of white flower color with mixed model of major gene plus polygene in Brassica napus L. Sci Agric Sin, 2009, 42:3987-3995 (in Chinese with English abstract).
[16] 刘雪平, 涂金星, 陈宝元, 傅廷栋. 人工合成甘蓝型油菜中花色与芥酸含量的遗传连锁分析. 遗传学报, 2004, 31:357-362.
Liu X P, Tu J X, Chen B Y, Fu T D. Identification of the linkage relationship between the flower colour and the content of erucic acid in the resynthesized Brassica napus L. Acta Genet Sin, 2004, 31:357-362 (in English with Chinese abstract).
[17] Han F Q, Yang C, Fang Z Y, Yang L M, Zhang M, Lyu H H, Liu Y M, Li Z S, Liu B, Yu H L, Liu X P, Zhang Y Y. cpc-1) in Brassica oleracea cpc-1) in Brassica oleracea. Mol Breed, 2015, 35:160.
doi: 10.1007/s11032-015-0354-x
[18] Huang Z, Ban Y Y, Bao R, Zhang X X, Xu A X, Ding J. Brassica napus L Brassica napus L. New Zealand J Crop Hortic Sci, 2014, 111:117.
[19] Zhang X X, Li R H, Niu S L, Chen L, Gao J, Wen J, Yi B, Ma C Z, Tu J X, Fu T D, Shen J X. Brassica juncea white-flowered mutant Bjpc2 using the whole-genome resequencing Brassica juncea white-flowered mutant Bjpc2 using the whole-genome resequencing. Mol Genet Genomics, 2017, 293:359-370.
doi: 10.1007/s00438-017-1390-5
[20] Xiao S, Xu J, Li Y, Zhang L, Shi S, Shi S, Wu J, Liu K. Brassica napus using a genome-walking technique Brassica napus using a genome-walking technique. Genome, 2007, 50:611-618.
doi: 10.1139/G07-044
[21] 陈雪, 王瑞, 井付钰, 张胜森, 贾乐东, 段谋正, 吴宇. 基于二代测序的甘蓝型油菜白花基因候选区间定位及连锁标记验证. 中国农业科学, 2020, 53:1108-1117.
Chen X, Wang R, Jing F Y, Zhang S S, Jia L D, Duan M Z, Wu Y. Location and linkage markers for candidate interval of the white petal gene in Brassica napus L. by next generation sequencing. Sci Agric Sin, 2020, 53:1108-1117 (in Chinese with English abstract).
[22] Zhang B, Liu C, Wang Y, Yao X, Wang F, Wu J, King G J, Liu K. Brassica species Brassica species. New Phytol, 2015, 206:1513-1526.
doi: 10.1111/nph.13335 pmid: 25690717
[23] Yao Y M, Li K X, Liu H D, Duncan R W, Guo S M, Xiao L, Du D Z. Bnpc 1) in spring Brassica napus L. to a 151-kb region Bnpc 1) in spring Brassica napus L. to a 151-kb region. Euphytica, 2017, 213:165.
doi: 10.1007/s10681-017-1959-4
[24] 丁戈, 陈伦林, 邹小云, 李书宇, 熊洁, 邹晓芬, 宋来强. 甘蓝型油菜桔黄花色基因的QTL-seq遗传分析及InDel分子标记开发. 分子植物育种, 2019, 17:3983-3992.
Ding G, Chen L L, Zou X Y, Li S Y, Xiong J, Zou X F, Song L Q. QTL-seq genetic analysis and InDel marker development of orange petel color gene in Brassica napus. Mol Plant Breed, 2019, 17:3983-3992 (in Chinese with English abstract).
[25] Mithra S V A, Kar M K, Mohapatra T, Robin S, Sarla N, Seshashayee M, Singh K, Singh N K, Sharma R P. DBT propelled national effort in creating mutant resource for functional genomics in rice. Curr Sci, 2016, 110:543-548.
doi: 10.18520/cs/v110/i4/543-548
[26] Wei F J, Droc G, Guiderdoni E, Hsing Y I C. International consortium of rice mutagenesis: Resources and beyond. Rice, 2013, 6:39.
doi: 10.1186/1939-8433-6-39
[27] Tsuda M, Kaga A, Anai T, Shimizu T, Sayamat, Takagi K, Machita K, Watanabe S, Nishimura M, Yamada N, Mori S, Sasaki H, Kanamori H, Katayose Y, Ishimoto M. Construction of a high-density mutant library in soybean and development of a mutant retrieval method using amplicon sequencing. BMC Genomics, 2015, 16:1014.
doi: 10.1186/s12864-015-2079-y pmid: 26610706
[28] Just D, Garcia V, Fernandez L, Bres C, Mauxion J P, Petit J, Jorly J, Assali J, Bournonville C, Ferrand C, Baldet P, Lemaire- Chamley M, Mori K, Okabe Y, Ariizumi T, Asamizu E, Ezura H, Rothan C. Micro-Tom mutants for functional analysis of target genes and discovery of new alleles in tomato. Plant Biotechnol J, 2013, 30:225-231.
[29] Lin T, Wang S H, Zhong Y, Gao D L, Cui Q Z, Chen H M, Zhang Z H, Shen H L, Weng Y Q, Huang S W. A truncated F-box protein confers the dwarfism in cucumber. J Genet Genomics, 2016, 43:223-226.
doi: 10.1016/j.jgg.2016.01.007
[30] Lun Y Y, Wang X, Zhang C Z, Yang L, Gao D L, Chen H M, Huang S W. A CsYcf54 variant conferring light green coloration in cucumber. Euphytica, 2016, 208:509-517.
doi: 10.1007/s10681-015-1592-z
[31] Zhou Q, Wang S H, Hu B W, Chen H M, Zhang Z H, Huang S W. An accumulation and replication of chloroplasts 5 gene mutation confers light green peel in cucumber. J Integr Plant Biol, 2015, 57:936-942.
doi: 10.1111/jipb.12355
[32] Takagi H, Abe A, Yoshid A K, Kosugi S, Natsume S, Mitsuoka C, Uemura A, Utsushi H, Tamiru M, Takumo S, Innan H, Cano L M, Kamoun S, Terauchi R. QTL-seq: rapid mapping of quantitative trait loci in rice by whole genome resequencing of DNA from two bulked populations. Plant J, 2013, 74:174-183.
doi: 10.1111/tpj.2013.74.issue-1
[33] Robert K, Ram Vinay P, Christian S. PoPoolation2: Identifying differentiation between populations using sequencing of pooled DNA samples (Pool-Seq). Bioinformatics, 2011, 27:3435-3436.
doi: 10.1093/bioinformatics/btr589 pmid: 22025480
[34] Hua Y P, Zhang D D, Zhou T, He M L, Ding G D, Shi L, Xu F S. Transcriptomics-assisted quantitative trait locus fine mapping for the rapid identification of a nodulin 26-like intrinsic protein gene regulating boron efficiency in allotetraploid rapeseed. Plant Cell Environ, 2016, 39:1601-1618.
doi: 10.1111/pce.v39.7
[35] 淡亚彬. 甘蓝型油菜桔红花色基因和心叶紫色基因的初步定位. 青海大学硕士学位论文, 青海西宁, 2016.
Dan Y B. Primary Mapping of the Orange Flower Gene and Central Leaf Color Gene in Brassica napus L. MS Thesis of Qinghai University, Xining, Qinghai, China, 2016 (in Chinese with English abstract)
[36] 潘存红, 王子斌, 马玉银, 殷跃军, 张亚芳, 左示敏, 陈宗祥, 潘学彪. InDel和SNP标记在水稻图位克隆中的应用. 中国水稻科学, 2007, 21:447-453.
Pan C H, Wang Z B, Ma Y Y, Yin Y J, Zhang Y F, Zuo S M, Chen Z X, Pan X B. InDel and SNP markers and their application in map-based cloning of rice genes. Chin J Rice Sci, 2007, 21:447-453 (in Chinese with English abstract).
[37] 胡坤. 玉米与大刍草InDel标记的开发及遗传连锁图谱的构建. 四川农业大学硕士学位论文, 四川雅安, 2014.
Hu K. The Development of Maize and Teosinte InDel Markers and Genetic Linkage Map Construction. MS Thesis of Sichuan Agricultural University, Ya’an, Sichuan, China, 2014 (in Chinese with English abstract).
[38] 吴迷, 汪念, 沈超, 黄聪, 温天旺, 林忠旭. 基于重测序的陆地棉InDel标记开发与评价. 作物学报, 2019, 45:196-203.
doi: 10.3724/SP.J.1006.2019.84100
Wu M, Wang N, Shen C, Huang C, Wen T W, Lin Z X. Development and evaluation of InDel markers in cotton based on whole-genome re-sequencing data. Acta Agron Sin, 2019, 45:196-203 (in Chinese with English abstract).
[39] 徐婷婷, 汪巧玲, 邹淑琼, 狄佳春, 杨欣, 朱银, 赵涵, 颜伟. 基于高通量测序的大麦InDel标记开发及应用. 作物学报, 2020, 46:1340-1355.
doi: 10.3724/SP.J.1006.2020.91076
Xu T T, Wang Q L, Zou S Q, Di J C, Yang X, Zhu Y, Zhao H, Yan W. Development and application of InDel markers based on high throughput sequencing in barley. Acta Agron Sin, 2020, 46:1340-1355 (in Chinese with English abstract).
[40] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状. 作物学报, 2021, 47:587-598.
doi: 10.3724/SP.J.1006.2021.04115
Zhou X T, Guo Q Q, Chen X, Li J N, Wang R. Construction of a high-density genetic map using genotyping by sequencing (GBS) for quantitative trait loci (QTL) analysis of pink petal trait in Brassica napus L. Acta Agron Sin, 2021, 47:587-598 (in Chinese with English abstract).
[1] 张超, 杨博, 张立源, 肖忠春, 刘景森, 马晋齐, 卢坤, 李加纳. 基于QTL定位和全基因组关联分析挖掘甘蓝型油菜收获指数相关位点[J]. 作物学报, 2022, 48(9): 2180-2195.
[2] 李胜婷, 徐远芳, 常玮, 刘亚俊, 谷嫄, 朱红, 李加纳, 卢坤. Bna.C02SWEET15通过光周期途径正向调控油菜开花时间[J]. 作物学报, 2022, 48(8): 1938-1947.
[3] 张天宇, 王越, 刘影, 周婷, 岳彩鹏, 黄进勇, 华营鹏. 油菜脯氨酸代谢基因家族的生物信息学分析与核心成员鉴定[J]. 作物学报, 2022, 48(8): 1977-1995.
[4] 戴丽诗, 常玮, 张赛, 钱明超, 黎小东, 张凯, 李加纳, 曲存民, 卢坤. Bna-novel-miR36421调节拟南芥株型和花器官发育的功能验证[J]. 作物学报, 2022, 48(7): 1635-1644.
[5] 秦璐, 韩配配, 常海滨, 顾炽明, 黄威, 李银水, 廖祥生, 谢立华, 廖星. 甘蓝型油菜耐低氮种质筛选及绿肥应用潜力评价[J]. 作物学报, 2022, 48(6): 1488-1501.
[6] 陈松余, 丁一娟, 孙峻溟, 黄登文, 杨楠, 代雨涵, 万华方, 钱伟. 甘蓝型油菜BnCNGC基因家族鉴定及其在核盘菌侵染和PEG处理下的表达特性分析[J]. 作物学报, 2022, 48(6): 1357-1371.
[7] 袁大双, 邓琬玉, 王珍, 彭茜, 张晓莉, 姚梦楠, 缪文杰, 朱冬鸣, 李加纳, 梁颖. 甘蓝型油菜BnMAPK2基因的克隆及功能分析[J]. 作物学报, 2022, 48(4): 840-850.
[8] 黄成, 梁晓梅, 戴成, 文静, 易斌, 涂金星, 沈金雄, 傅廷栋, 马朝芝. 甘蓝型油菜BnAPs基因家族成员全基因组鉴定及分析[J]. 作物学报, 2022, 48(3): 597-607.
[9] 王艳花, 刘景森, 李加纳. 整合GWAS和WGCNA筛选鉴定甘蓝型油菜生物产量候选基因[J]. 作物学报, 2021, 47(8): 1491-1510.
[10] 李杰华, 端群, 史明涛, 吴潞梅, 柳寒, 林拥军, 吴高兵, 范楚川, 周永明. 新型抗广谱性除草剂草甘膦转基因油菜的创制及其鉴定[J]. 作物学报, 2021, 47(5): 789-798.
[11] 唐鑫, 李圆圆, 陆俊杏, 张涛. 甘蓝型油菜温敏细胞核雄性不育系160S花药败育的形态学特征和细胞学研究[J]. 作物学报, 2021, 47(5): 983-990.
[12] 周新桐, 郭青青, 陈雪, 李加纳, 王瑞. GBS高密度遗传连锁图谱定位甘蓝型油菜粉色花性状[J]. 作物学报, 2021, 47(4): 587-598.
[13] 李书宇, 黄杨, 熊洁, 丁戈, 陈伦林, 宋来强. 甘蓝型油菜早熟性状QTL定位及候选基因筛选[J]. 作物学报, 2021, 47(4): 626-637.
[14] 张春, 赵小珍, 庞承珂, 彭门路, 王晓东, 陈锋, 张维, 陈松, 彭琦, 易斌, 孙程明, 张洁夫, 傅廷栋. 甘蓝型油菜千粒重全基因组关联分析[J]. 作物学报, 2021, 47(4): 650-659.
[15] 唐婧泉, 王南, 高界, 刘婷婷, 文静, 易斌, 涂金星, 傅廷栋, 沈金雄. 甘蓝型油菜SnRK基因家族生物信息学分析及其与种子含油量的关系[J]. 作物学报, 2021, 47(3): 416-426.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
[1] 李绍清, 李阳生, 吴福顺, 廖江林, 李达模. 水稻孕穗期在淹涝胁迫下施肥的优化选择及其作用机理[J]. 作物学报, 2002, 28(01): 115 -120 .
[2] 王兰珍;米国华;陈范骏;张福锁. 不同产量结构小麦品种对缺磷反应的分析[J]. 作物学报, 2003, 29(06): 867 -870 .
[3] 王艳;邱立明;谢文娟;黄薇;叶锋;张富春;马纪. 昆虫抗冻蛋白基因转化烟草的抗寒性[J]. 作物学报, 2008, 34(03): 397 -402 .
[4] 郑希;吴建国;楼向阳;徐海明;石春海. 不同环境条件下稻米组氨酸和精氨酸的胚乳和母体植株QTL分析[J]. 作物学报, 2008, 34(03): 369 -375 .
[5] 邢光南, 周斌, 赵团结, 喻德跃, 邢邯, 陈受宜, 盖钧镒. 大豆抗筛豆龟蝽Megacota cribraria (Fabricius)的QTL分析[J]. 作物学报, 2008, 34(03): 361 -368 .
[6] 郑永美;丁艳锋;王强盛;李刚华;王惠芝;王绍华. 起身肥对水稻分蘖和氮素吸收利用的影响[J]. 作物学报, 2008, 34(03): 513 -519 .
[7] 秦恩华;杨兰芳. 烤烟苗期含硒量和根际硒形态的研究[J]. 作物学报, 2008, 34(03): 506 -512 .
[8] 吕丽华;陶洪斌;夏来坤; 张雅杰; 赵明; 赵久然;王璞. 不同种植密度下的夏玉米冠层结构及光合特性[J]. 作物学报, 2008, 34(03): 447 -455 .
[9] 张书标;杨仁崔. e-杂交稻若干生物学特性研究[J]. 作物学报, 2003, 29(06): 919 -924 .
[10] 邵瑞鑫;上官周平. 外源一氧化氮供体SNP对受旱小麦光合色素含量和PS II光能利用能力的影响[J]. 作物学报, 2008, 34(05): 818 -822 .