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作物学报 ›› 2009, Vol. 35 ›› Issue (9): 1590-1596.doi: 10.3724/SP.J.1006.2009.01590

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

优良大豆品种合丰25的遗传组成

关荣霞1,秦君2,胡静深3,陈为秀3,常汝镇1,刘章雄1,邱丽娟1,*   

  1. 1中国农业科学院作物科学研究所/高家农作物基因资源与基因改良重大科学工程/农业部作物种质资源利用重点开放实验室，北京100081；2河北农林科学院粮油作物研究所，河北石家庄050031；3东北农业大学，黑龙江哈尔滨 150030
  • 收稿日期:2009-01-08 修回日期:2009-04-26 出版日期:2009-09-12 网络出版日期:2009-07-03
  • 通讯作者: 邱丽娟, E-mail: qiu_lijuan@263.net; Tel: 010-82105843
  • 基金资助:

    本研究由国家高科技研究发展计划（863计划）项目（2006AA100104，2006AA10B1Z3，2006AA10Z1F1），国家自然科学基金项目（30490251，30671310），国家科技攻关项目（2006BAB13B05），国家重点基础研究发展计划（973计划）项目（2009CB118400）资助。

Genetic Composition of Elite Soybean Dultivar Hefeng 25

GUAN Rong-Xia1,QIN Jun2,HU Jing-Shen3,CHEN Wei-Xiu3,CHANG Ru-Zhen1,LIU Zhang-Xiong,QIU Li-Juan1   

  1. 1 National Key Facility for Crop Gene Resources and Genetic Improvement / Key Laboratory of Germplasm Utilization, Ministry of Agriculture / Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Hebei Academy of Agricultural and Forestry Science, Shijiazhuang 050031, China; 3 Northeast Agricultural University, Harbin 150030, China
  • Received:2009-01-08 Revised:2009-04-26 Published:2009-09-12 Published online:2009-07-03
  • Contact: QIU Li-Juan, E-mail: qiu_lijuan@263.net; Tel: 010-82105843

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3261

被引次数

9

摘要：

合丰25由合丰23和克4430-20杂交选育而成，是我国大豆生产上累计种植面积最大的品种。本研究利用463个SSR标记对合丰25及其亲本进行检测，并分析其遗传组成及其与亲本的遗传关系。463个SSR位点中有177个位点在合丰25的两个亲本间无多态性，合丰23和克4430-20对合丰25的遗传贡献率分别为39.4%和48.3%。在G和E两个连锁群发现克4430-20有大片段传递给合丰25，特别是G连锁群没有发现来自合丰23的片段，而合丰23在L连锁群传递给合丰25的位点是克4430-20的2.3倍。不同连锁群亲本染色体片段的组成不同，可能与产量、抗病性等重要性状相关QTL的分布有关，从而使合丰25通过重组集合了两个亲本的优点。合丰25在57个位点出现新等位变异。为分析位点突变对合丰25纯度的影响，用包括57个位点在内的207个SSR标记对12个不同销售点的合丰25种子进行检测，207对SSR引物中有13对在4个大豆样本中检测到杂合条带，而8个样本的纯度均为100%。说明大部分突变位点已在品种利用过程中纯合，合丰25的优异基因型及其在推广20多年后仍保持的高纯度，是其在生产上利用经久不衰的主要原因之一。

关键词: 大豆, 合丰25, 系谱, SSR, 遗传组成

Abstract:

Elite soybean cultivar Hefeng 25, derived from a cross of Hefeng 23×Ke 4430-20, has an accumulated planting area of 13 million hectares and the longest planting history of soybean cultivars. The objective of this study is to evaluate the genetic composition of Hefeng 25 and the relationship of Hefeng 25 with the parents. Four hundred and sixty-three SSR markers were used to screen Hefeng 25 and its parents Hefeng 23 and Ke 4430-20. Of the 463 SSR markers 177 were monomorphic between Hefeng 23 and Ke 4430-20 and 57 loci were detected in Hefeng 25 with new alleles mutated not from the parents. The genetic contribution of Hefeng 23 and Ke 4430-20 to Hefeng 25 was 39.4% and 48.3%, respectively based on molecular information. Analysis of each linkage group revealed that large portions of loci on linkage groups G, E, and L were inherited entirely from one parent. Especially on linkage group G, all loci were inherited from Ke4430-20. The loci on linkage group L inherited from Hefeng 23 were 2.3 fold of that from Ke4430-20. Detailed analysis showed that QTLs for yield and disease resistance may relate to these loci. These analyses suggested that breeders may select recombination events with agronomic favorable alleles of two parents. In order to evaluate the effect of SSR mutation on soybean seed purity, we screened Hefeng 25 seed samples from 12 different seed companies at 207 SSR loci including 57 mutated loci. Hybrid alleles were observed only in 4 seed samples at 13 SSR loci. The result indicated that most of the mutated loci were purified during the breeding process. The elite genotype and high seed purity of Hefeng25 may be the most important factors for its long-term utilization in soybean production.

Key words: Soybean(Glycine max), Hefeng 25, Pedigree, SSR, Genetic composition

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