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作物学报 ›› 2011, Vol. 37 ›› Issue (10): 1904-1909.doi: 10.3724/SP.J.1006.2011.01904

• 研究简报 • 上一篇    下一篇

水稻种胚特异性启动子OsESP1的克隆及其表达特性分析

房孝良1,2,3，刘炜1,2,3,*，安静1，王庆国1,2   

  1. 1山东省农业科学院高新技术研究中心 / 山东省作物遗传改良与生物技术重点实验室, 山东济南 250100; 2农业部黄淮海作物遗传改良与生物技术重点开放实验室, 山东济南 250100; 3山东师范大学生命科学学院, 山东济南250014
  • 收稿日期:2011-03-07 修回日期:2011-06-25 出版日期:2011-10-12 网络出版日期:2011-07-28
  • 通讯作者: 刘炜, E-mail: wheiliu@163.com, Tel: 0531-83179572

Isolation and Characterization of an Embryo-specific Promoter OsESP1 from Rice

FANG Xiao-Liang1,2,3,LIU Wei1,2,3,*,AN Jing1,WANG Qing-Guo1,2   

  1. 1 High-Tech Research Center, Shandong Academy of Agricultural Sciences / Key Laboratory for Genetic Improvement of Crop Animal and Poultry of Shandong Province, Jinan 250100, China; 2 Key Laboratory of Crop Genetic Improvement and Biotechnology, Huanghuaihai, Ministry of Agriculture, Jinan 250100, China; 3 College of Life Sciences, Shandong Normal University, Jinan 250014, China
  • Received:2011-03-07 Revised:2011-06-25 Published:2011-10-12 Published online:2011-07-28
  • Contact: 刘炜, E-mail: wheiliu@163.com, Tel: 0531-83179572

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1491

被引次数

9

摘要： 以水稻品种“中花11”基因组DNA为模板，通过PCR的方法，对水稻基因OsESG上游调控序列扩增，获得长度约为1.4 kb 的特异性条带，命名为OsESP1。以OsESP1与带有GUS报告基因的植物表达载体连接，经农杆菌介导转化获得转基因水稻阳性植株。结合组织化学染色法，检测GUS报告基因的表达特性，结果表明，由OsESP1所调控的GUS报告基因仅在水稻种胚中特异表达，而在其他组织中均未被检测到，初步证明OsESP1为水稻种胚特异性启动子。

关键词: 水稻, 种胚特异性启动子, 表达特性分析, GUS报告基因

Abstract: A pair of specific primers were designed according to the 5′ upstream regulatory sequence of rice gene OsESG, anda 1.4 kb fragement named OsESP1was amplified using rice (Oryza sativa L. cv. Zhonghua 11) genomic DNA as template by PCR. OsESP1 was further fused with GUS reporter gene and transformed into rice callus through Agrobacterium-mediated transformation. GUS activities in various tissues of transformed plants were examined and the GUS activity was detected only in rice embryo, indicating theOsESP1 is an embryo-specific promoter.

Key words: Rice, Embryo-specific promoter, Expression patern analysis, GUS reporter gene

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