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作物学报 ›› 2012, Vol. 38 ›› Issue (11): 2108-2114.doi: 10.3724/SP.J.1006.2012.02108

• 耕作栽培·生理生化 • 上一篇    下一篇

甘蓝型油菜DH群体苗期抗旱性的评价

李真2,梅淑芳2,梅忠2,刘芳1,周广生1*, 吴江生1   

  1. 1 华中农业大学植物科学技术学院,湖北武汉 430070;2 金华职业技术学院农业与生物工程学院,浙江金华 321000
  • 收稿日期:2012-03-18 修回日期:2012-07-05 出版日期:2012-11-12 网络出版日期:2012-09-10
  • 通讯作者: 周广生, E-mail: zhougs@mail.hzau.edu.cn, Tel: 027-87281507
  • 基金资助:

    本研究由国家公益性行业(农业)科研专项(201203096), 国家科技支撑项目(2010BAD01B09)和国家现代农业产业技术体系建设项目(CARS-13)。

Evaluation of Drought Resistance in Rapeseed (Brassica napus L.) DH Lines at Seedling Stage

LI Zhen2,MEI Shu-Fang2,MEI Zhong2,LIU Fang1,ZHOU Guang-Sheng1,*,WU Jiang-Sheng1   

  1. 1 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2 Department of Agriculture and Bioengineering, Jinhua College of Profession and Technology, Jinhua 321000, China
  • Received:2012-03-18 Revised:2012-07-05 Published:2012-11-12 Published online:2012-09-10
  • Contact: 周广生, E-mail: zhougs@mail.hzau.edu.cn, Tel: 027-87281507

摘要:

中国油菜主产区常常受到秋冬旱和春旱危害而影响产量及品质。本文通过盆栽试验,以株高(PH)、根长(RL)、地上部干重(SDW)、根干重(RDW)、根冠比(R/S)、总干重(TDW) 6个性状的抗旱系数作为抗旱性评价指标,对甘蓝型油菜DH(doubled haploid)群体的118个株系及其亲本进行苗期抗旱性评价,筛选极端抗旱DH系。结果表明,与对照相比,干旱严重抑制了甘蓝型油菜苗期的生长,6个评价指标均表现出显著差异,其中根干重的变异系数最大;在对照和干旱条件下,群体各株系各性状均表现出超亲连续分离,大部分呈正态分布,抗旱条件下的分离更为明显;相关性分析表明,地上部干重、根干重和总干重抗旱系数可作为甘蓝型油菜苗期抗旱性的主要评价指标;

关键词: 甘蓝型油菜, 苗期, 抗旱性, 评价指标

Abstract:

Drought stress in autumn, winter and spring has endangered the production and quality of rapeseed in China.The objective of the study was to evaluate drought resistance of the rapeseed (Brassica napus L.) DH (doubled haploid) population and screen the most drought-resistant DH lines. For phenotyping, the rapeseed lines from DH population [(Huyou 15×5900) F4 × (No2127-17×275B) F4] were grown in plastic pots. Six traits including plant height, root length, shoot dry weight (SDW), root dry weight (RDW), root/shoot ratio and total dry weight (TDW) together with DRC (drought resistance coefficient) of all the traits were used to evaluate the drought resistance of the 118 DH lines and their parents. The results showed that the six responsive traits changed significantly under the drought conditions compared with the control in the experiment and the effects of drought were much more severe on RDW than on other traits. All traits showed transgressive and continuous distribution under both drought and control conditions. The correlation among all the traits investigated in the study indicated that DRC of SDW, RDW and TDW could be used as available evaluation indices for drought resistance. Lines 032, 034, and 035 screened from the population were the most drought-resistant lines and 006, 091, and 104 were the most drought-sensitive lines.

Key words: Brassica napus, Seedling stage, Drought resistance, Evaluation index

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