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作物学报 ›› 2016, Vol. 42 ›› Issue (11): 1638-1646.

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

粳稻品种吉粳809的稻瘟病抗性基因分析

朱亚军1,**,孙强2,**,王金明2,陈凯1,冯博1,方雅洁1,林秀云2,*,徐建龙1,3,*   

  1. 1中国农业科学院作物科学研究所,北京100081;2吉林省农业科学院水稻研究所,吉林公主岭136100;3中国农业科学院深圳生物育种创新研究院,广东深圳518120
  • 收稿日期:2016-08-01 修回日期:2016-08-02 出版日期:2016-11-12 网络出版日期:2016-08-11
  • 通讯作者: 徐建龙,E-mail:xujlcaas@126.com,Tel:010-82105854;林秀云,E-mail:linxiuyun1965@163.com,Tel:13504462960
  • 基金资助:

    本研究由引进国际先进农业科学技术计划(948计划)项目(2010-G2B-2),吉林省科技成果转化计划项目(20130305038NY),国家高技术研究发展计划(863计划)项目(2014AA10A601)和吉林省自然科学基金项目(20140101014JC)资助。

Analysisof Blast Resistance Genesin Japonica Variety Jijing 809

ZHU Ya-Jun1,**, SUN Qiang2,**, WANG Jin-Ming2, CHEN Kai1, FENG Bo1, FANG Ya-Jie1, LIN Xiu-Yun2,*, and XU Jian-Long1,3,*   

  1. 1 National Facility for Crop Gene Resources and Genetic Improvement / Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2 Rice Research Institute, Jilin Academy of agricultural Sciences, Gongzhuling 130000, China; 3 Shenzhen Institute of Breeding and Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
  • Received:2016-08-01 Revised:2016-08-02 Published:2016-11-12 Published online:2016-08-11
  • Contact: 徐建龙,E-mail:xujlcaas@126.com,Tel:010-82105854;林秀云,E-mail:linxiuyun1965@163.com,Tel:13504462960
  • Supported by:

    ThisstudywassupportedbytheProgramofIntroducingInternationalSuperAgriculturalScienceandTechnology(948Program,2010-G2B-2),ProjectoftransformationofscientificandtechnologicalachievementsinJilinProvince(20130305038NY),theNationalHighTechnologyResearchandDevelopmentProgramofChina(863Program,2014AA10A601),andNaturalScienceFoundationofJilinProvince(20140101014JC).

摘要:

稻瘟病是水稻生产中危害最为严重的病害之一,种植抗病品种是抵御稻瘟病危害的有效措施。本研究利用吉粳809的2个亲本材料吉粳88与93072配制的回交分离群体进行稻瘟病人工接种,采用抗、感极端分池法定位双亲的稻瘟病抗性基因,结合基因型分析,推断吉粳809的抗性基因组成。结果表明,回交群体对强致病菌GD9-1表现为4个主效抗病基因Pi-2(t)、Pi-7-1(t)、Pi-7-2(t)和Pi-11(t)分离,对弱致病菌GD19-1表现单个主效抗病基因Pi-2(t)分离,其中Pi-2(t)基因同时抗2个菌株。除Pi-2(t)位点抗性等位基因来自吉粳88,其余3个位点的抗性等位基因均来自93072。比较基因组研究表明,Pi-2(t)可能与Pi-b等位,Pi-11(t)可能与Pi-47(t)或Pik等位,而Pi-7-1(t)和Pi-7-2(t)是2个新的抗性位点,分别与RM21260和RM8037连锁,遗传距离为0.11 cM和6.97 cM。利用与上述4个抗病位点紧密连锁的SSR标记和来自3K水稻种质资源重测序开发的55K芯片鉴定抗性位点吉粳809与双亲基因型的异同,推断吉粳809抗性基因组成,发现吉粳809携带轮回亲本吉粳88的Pi-2(t)和来自供体亲本93072的Pi-11(t) 2个基因,合理地解释了吉粳809抗性明显好于吉粳88的原因。对如何通过不同主效抗性基因聚合特别是充分利用原来抗病品种中“丧失”抗性残效基因来改良品种的稻瘟病抗性进行了讨论。

关键词: 水稻, 稻瘟病, 抗病基因, 极端分离群体分池法, 抗性基因聚合

Abstract:

Blast is one of the most hazardous diseases in rice production. Planting resistance variety is an effective measure to control the disease. In this study, a backcross population derived from two parents, Jijing 88 and 93072, was selected to identify blast resistance genes using bulked extremes and recessive class under artificial inoculation and further to deduce the composition of resistance genes in Jijing 809 developed from Jijing 88 and 93072. Four major resistance genes including Pi-2(t), Pi-7-1(t), Pi-7-2(t), and Pi-11(t) were responsible for segregation of resistance to the strong virulent strain GD9-1, and only one resistance gene Pi-2(t) for segregation of resistance to the weak virulent strain GD19-1. Among them, Pi-2(t) was effective to the two strains. The favorable alleles at all loci were from 93072 except for the allele at Pi-2(t) from Jijing 88. By comparative mapping, Pi-2(t) was deduced to be allelic to Pi-b, and Pi-11(t) to Pi-47(t) or Pik, whereas Pi-7-1(t) and Pi-7-2(t) were the two newly identified resistant genes, having linkages with RM21260 and RM8037 at 0.11 and 6.97 cM, respectively. SSR markers tight by linked with the four resistance genes mentioned above and a set of 55K gene chip developed from re-sequenced data of 3K rice germplasm were used to compare and identify genotypes of Jijing 809 at the above resistant loci with the two parents. It was indicated that two pairs of resistant genes, Pi-2(t) and Pi-11(t), in Jijing 809 were from the recurrent parent Jijing 88 and the donor parent 93072, respectively, which reasonably explained that Jijing 809 had much better blast resistance than Jijing 88. It was discussed how to pyramid different known major resistant genes, especially to make full use of the ‘defeated’ resistance gene in the original resistant variety to improve blast resistance of rice.

Key words: Rice, Blast, Resistance gene, Bulked extremes and recessive class, Pyramiding of resistance gene

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