欢迎访问作物学报,今天是

作物学报 ›› 2017, Vol. 43 ›› Issue (01): 1-8.doi: 10.3724/SP.J.1006.2017.00001

• 作物遗传育种·种质资源·分子遗传学 •    下一篇

菜豆普通细菌性疫病抗性基因定位

朱吉风,武晶,王兰芬,朱振东,王述民*   

  1. 中国农业科学院作物科学研究所,北京100081
  • 收稿日期:2016-05-04 修回日期:2016-07-11 出版日期:2017-01-12 网络出版日期:2016-07-28
  • 通讯作者: 王述民. E-mail: wangshumin@caas.cn
  • 基金资助:

    本研究由国家现代农业产业技术体系建设专项(CARS-09),国家科技支撑计划项目(2013BAD01B03-18a)和中国农业科学院科技创新工程项目资助。

Mapping of Common Bacterial Blight Resistance Gene in Common Bean

ZHU Ji-Feng,WU Jing,WANG Lan-Fen,ZHU Zhen-Dong,WANG Shu-Min   

  1. Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  • Received:2016-05-04 Revised:2016-07-11 Published:2017-01-12 Published online:2016-07-28
  • Contact: 王述民. E-mail: wangshumin@caas.cn
  • Supported by:

    This study was supported by the China Agriculture Research System (CARS-09), the National Key Technology R&D Program of China (2013BAD01B03-18a), and the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences.

摘要:

菜豆普通细菌性疫病是世界上危害普通菜豆生产的最严重的病害之一。龙芸豆5号是我国黑龙江省的主栽品种,对菜豆普通细菌性疫病表现出良好的抗性。为定位来源于龙芸豆5号的抗性基因,本研究构建了包含785个单株的F2分离群体。基于该群体构建了一张包含206个SSR标记,总长度1648.42 cM,标记间平均遗传距离8.00 cM的遗传图谱。图谱包含12个连锁群,各连锁群平均长度137.37 cM,连锁群上标记数量3~35个。结合温室表型鉴定结果,采用QTL IciMapping v4.0软件的完备区间作图法进行QTL定位和效应估计。接种14 d后在Pv06染色体上检测到一个抗病QTL。该位点位于标记p6s249p6s183之间,加性效应值为0.44,说明增效基因来源于龙芸豆5号,LOD值为5.93,表型贡献率为4.61%,该抗病QTL的效应值相对较低,将在培育稳定持久的抗菜豆普通细菌性疫病的品种中发挥作用。最后,对抗性基因紧密连锁的11对SSR引物与菜豆普通细菌性疫病抗性的关联分析表明,SSR标记p6s249与菜豆普通细菌性疫病抗性极显著关联(P<0.001),该标记可用于抗病分子育种。

关键词: 普通菜豆, 普通细菌性疫病, SSR, QTL, 关联分析

Abstract:

Common bacterial blight disease is a serious disease affecting the production of common bean (Phaseolus vulgaris L.) worldwide. The common bean germplasm Longyundou 5 is the main cultivar carrying common bacterial blight resistance genein Heilongjiang province. To study the genetic mechanisms behind this resistance, we constructed 785 F2 plants from Longyundou 5. Linkage analysis was performed on this population by using SSR markers. A linkage map covering 1648.42 cM with an average marker distance of 8.00 cM among 206 SSR markers was constructed. This map contained 12 linkage groups with a mean group length of 137.37 cM and the number of loci ranging from three to thirty-five. Combined with the results of phenotypic evaluation, QTL analysis was performed by the inclusive composite interval mapping method with QTL IciMapping v4.0. A QTL was detected between p6s249 and p6s183 on chromosome Pv06, with an additive effect of 0.44, which means the favourable gene of this locus is from Longyundou 5. The LOD score of the QTL was 5.93 and the total phenotypic variation at 14 days after inoculation was 4.61%. These results showed the effect of the QTL was low, which will play an important role in breeding durable resistant varieties of common bean. Association analysis between 11 SSR primers linked with common bacterial blight resistance and the disease rating showed that SSR marker p6s249 is excellently associated (P<0.001) with common bacterial blight resistance and can be used for marker assisted selection.

Key words: Phaseolus vulgaris L, Common bacterial blight, SSR, QTL, Association analysis

[1] Bitocchi E, Nanni L, Bellucci E, Rossi M, Giardini A, Zeuli P S, Logozzo G, Stougaard J, McClean P, Attene G, Papa R. Mesoamerican origin of the common bean (Phaseolus vulgaris L.) is revealed by sequence data. Proc Natl Acad Sci USA, 2012, 109: E788–E796
[2] Schuster M L, Coyne D P. Biology, epidemiology, genetics and breeding for resistance to bacterial pathogens of Phaseolus vulgaris L. Hort Rev, 1981, 3: 28–58
[3] Singh S P, Schwartz H F. Breeding common bean for resistance to diseases: a review. Crop Sci, 2010, 50: 2199–2223
[4] Lema-Marquez M, Teran H, Singh S P. Selecting common bean with genes of different evolutionary origins for resistance to Xanthomonas campestris pv. phaseoli. Crop Sci, 2007, 47: 1367–1374
[5] Jung G, Skroch P W, Nienhuis J, Coyne D P, Arnaud-Santana E, Ariyarathne H M, Marita J M. Confirmation of QTL associated with common bacterial blight resistance in four different genetic backgrounds in common bean. Crop Sci, 1999, 39: 1448–1455
[6] Miklas P N, Kelly J D, Beebe S E, Blair M W. Common bean breeding for resistance against biotic and abiotic stresses: from classical to MAS breeding. Euphytica, 2006, 147: 105–131
[7] Tar’an B, Michaels T E, Pauls K P, Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions. Genome, 2001, 44: 1046–1056
[8] Liu S, Yu K, Park S J. Development of STS markers and QTL validation for common bacterial blight resistance in common bean. Plant Breed, 2008, 127: 62–68
[9] Shi C, Navabi A, Yu K. Association mapping of common bacterial blight resistance QTL in Ontario bean breeding populations. BMC Plant Biol, 2011, 11: 52
[10] Jung G, Coyne D P, Skroch P W, Nienhuis J, Arnaud-Santana E, Bokosi J, Ariyarathne H M, Steadman J R, Beaver J S, Kaeppler S M. Molecular markers associated with plant architecture and resistance to common blight, web blight, and rust in common beans. J Am Soc Hort Sci, 1996, 121: 794–803
[11] Ariyarathne H M, Coyne D P, Jung G, Skroch P W, Vidaver A K, Steadman J R, Miklas P N, Bassett M. Molecular mapping of disease resistance genes for halo blight, common bacterial blight, and bean common mosaic virus in a segregating population of common bean. J Am Soc Hort Sci, 1999, 124: 654–662
[12] Yu K, Park S J, Zhang B, Haffner M, Poysa V. An SSR marker in the nitrate reductase gene of common bean is tightly linked to a major gene conferring resistance to common bacterial blight. Euphytica, 2004, 138: 89–95
[13] Miklas P N, Coyne D, Grafton K F, Mutlu N, Reiser J, Lindgren D T, Singh S P. A major QTL for common bacterial blight resistance derives from the common bean great northern landrace cultivar Montana No.5. Euphytica, 2003, 131: 137–146
[14] Pedraza F, Gallego G, Beebe S, Tohme J. Marcadores SCAR y RAPD para la resistencia a la bacteriosis comun (CBB). In: Singh S P, Voysest O, eds. Taller de Mejoramiento de frijol para el Siglo XXI: Bases Para Una Estrategia Para America Latina. CIAT Cali Colombia, 1997. pp 130–134
[15] Thomas C V, Waines J G. Fertile backcross and allotetraploid plants from crosses between tepary beans and common beans. J Hered, 1984, 75: 93–98.
[16] Vandemark G J, Fourie D, Miklas P N. Genotyping with real-time PCR reveals recessive epistasis between independent QTL conferring resistance to common bacterial blight in dry bean. Theor Appl Genet, 2008, 117: 513–522
[17] Shi C, Yu K, Xie W, Perry G, Navabi A, Pauls K P, Miklas P N, Fourie D. Development of candidate gene markers associated to common bacterial blight resistance in common bean. Theor Appl Genet, 2012, 125: 1525–1537
[18] Sheppard J W, Kurowski C, Remeeus P M. International Rules for seed testing, 7-021: Detection of Xanthomonas axonopodis pv. phaseoli and Xanthomonas axonopodis pv. phaseoli var. fuscans on Phaseolus vulgaris. International Seed Testing Association (ISTA), Bassersdorf, Switzerland. 2007, http://www.seedtest.org/upload/cms/user/7-021.pdf
[19] Zapata M. Proposed of a uniform screening procedure for the evaluation of variability of Xanthomonas axonopodis pv. phaseoli and resistance on leaves of Phaseolus vulgaris under greenhouse conditions. Annu Rep Bean Improv Coop, 2006, 49: 213–214
[20] Zapata M, Beaver J S, Porch T G. Dominant gene for common bean resistance to common bacterial blight caused by Xanthomonas axonopodis pv. phaseoli. Euphytica, 2011, 179: 373–382
[21] Afanador L K, Hadley S D, Kelly J D. Adoption of a “mini-prep” DNA extraction method for RAPD marker analysis in common bean (Phaseolus vulgaris L.). Annu Rep Bean Improv Coop, 1993, 36: 10–11
[22] Hanai L R, Santini L, Camargo L E A, Fungaro M H P, Gepts P, Tsai S M, Vieira M L C. Extension of the core map of common bean with EST-SSR, RGA, AFLP, and putative functional markers. Mol Breed, 2010, 25: 25–45
[23] Chen M, Wu J, Wang L, Zhang X, Blair M W, Jia J, Wang S. Development of mapped simple sequence repeat markers from common bean (Phaseolus vulgaris L.) based on genome sequences of a Chinese landrace and diversity evaluation. Mol Breed, 2014, 33: 489–496
[24] Schmutz J, McClean P E, Mamidi S, Wu G A, Cannon S B, Grimwood J, Jenkins J, Shu S, Song Q, Chavarro C, Torres-Torres M, Geffroy V, Moghaddam S M, Gao D, Abernathy B, Barry K, Blair M, Brick M A, Chovatia M, Gepts P, Goodstein D M, Gonzales M, Hellsten U, Hyten D L, Jia G, Kelly J D, Kudrna D, Lee R, Richard M M, Miklas P N, Osorno J M, Rodrigues J, Thareau V, Urrea C A, Wang M, Yu Y, Zhang M, Wing R A, Cregan P B, Rokhsar D S, Jackson S A. A reference genome for common bean and genome-wide analysis of dual domestications. Nat Genet, 2014, 46: 706–713
[25] Zhang C L, Wang Y, Chen H, Lan X Y, Lei C Z. Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining. Anal Biochem, 2007, 365: 286–287
[26] Meng L, Li H H, Zhang L Y, Wang J K. QTL IciMapping: Integrated software for genetic linkage map construction and quantitative trait locus mapping in biparental populations. Crop J, 2015, 3: 269– 283
[27] Kosambi D D. The estimation of map distances from recombination values. Ann Eugen, 1943, 12: 172–175
[28] Li H, Ye G, Wang J, A modified algorithm for the improvement of composite interval mapping. Genetics, 2007, 175: 361–374
[29] Bradbury P, Zhang Z, Kroon D E, Casstevens T M, Ramdoss Y, Buckler E S. TASSEL: software for association mapping of complex traits in diverse samples. Bioinformatics, 2007, 23: 2633–2635
[30] Ribaut J M, Hoisington D. Marker-assisted selection: New tools and strategies. Trends Plant Sci, 1998, 3: 236–239
[31] Toojinda T, Tragoonrung S, Vanavichit A, Siangliw J L, Pa-In N, Jantaboon J, Siangliw M, Fukai S. Molecular breeding for rainfed lowland rice in the mekong region. Plant Prod Sci, 2005, 8: 330–333
[32] Abalo G, Tongoona P, Derera J, Edema R. A comparative analysis of conventional and marker-assisted selection methods in breeding maize streak virus resistance in maize. Crop Sci, 2009, 49: 509–520
[33] Gupta P K, Langridge P, Mir R R. Marker-assisted wheat breeding: present status and future possibilities. Mol Breed, 2010, 26: 145–161
[34] Collard B C, Mackill D J. Marker-assisted selection: an approach for precision plant breeding in the twenty-first century. Philos Trans R Soc Lond, 2008, 363: 557–572
[35] O’Boyle P D, Kelly J D, Kirk W W. Use of marker-assisted selection to breed for resistance to common bacterial blight in common bean. J Am Soc Hort Sci, 2007, 132: 381–386

[1] 陈玲玲, 李战, 刘亭萱, 谷勇哲, 宋健, 王俊, 邱丽娟. 基于783份大豆种质资源的叶柄夹角全基因组关联分析[J]. 作物学报, 2022, 48(6): 1333-1345.
[2] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[3] 孙思敏, 韩贝, 陈林, 孙伟男, 张献龙, 杨细燕. 棉花苗期根系分型及根系性状的关联分析[J]. 作物学报, 2022, 48(5): 1081-1090.
[4] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[5] 陈小红, 林元香, 王倩, 丁敏, 王海岗, 陈凌, 高志军, 王瑞云, 乔治军. 基于高基元SSR构建黍稷种质资源的分子身份证[J]. 作物学报, 2022, 48(4): 908-919.
[6] 张霞, 于卓, 金兴红, 于肖夏, 李景伟, 李佳奇. 马铃薯SSR引物的开发、特征分析及在彩色马铃薯材料中的扩增研究[J]. 作物学报, 2022, 48(4): 920-929.
[7] 黄莉, 陈玉宁, 罗怀勇, 周小静, 刘念, 陈伟刚, 雷永, 廖伯寿, 姜慧芳. 花生种子大小相关性状QTL定位研究进展[J]. 作物学报, 2022, 48(2): 280-291.
[8] 渠建洲, 冯文豪, 张兴华, 徐淑兔, 薛吉全. 基于全基因组关联分析解析玉米籽粒大小的遗传结构[J]. 作物学报, 2022, 48(2): 304-319.
[9] 张艳波, 王袁, 冯甘雨, 段慧蓉, 刘海英. 棉籽油分和3种主要脂肪酸含量QTL分析[J]. 作物学报, 2022, 48(2): 380-395.
[10] 赵海涵, 练旺民, 占小登, 徐海明, 张迎信, 程式华, 楼向阳, 曹立勇, 洪永波. 水稻协优9308重组自交系群体白叶枯病抗性的全基因组关联分析[J]. 作物学报, 2022, 48(1): 121-137.
[11] 许德蓉, 孙超, 毕真真, 秦天元, 王一好, 李成举, 范又方, 刘寅笃, 张俊莲, 白江平. 马铃薯StDRO1基因的多态性鉴定及其与根系性状的关联分析[J]. 作物学报, 2022, 48(1): 76-85.
[12] 于芮苏, 田小康, 刘斌斌, 段迎新, 李婷, 张秀英, 张兴华, 郝引川, 李勤, 薛吉全, 徐淑兔. 玉米抗倒伏相关性状QTL的关联和连锁分析[J]. 作物学报, 2022, 48(1): 138-150.
[13] 张波, 裴瑞琴, 杨维丰, 朱海涛, 刘桂富, 张桂权, 王少奎. 利用单片段代换系鉴定巴西陆稻IAPAR9中的粒型基因[J]. 作物学报, 2021, 47(8): 1472-1480.
[14] 耿腊, 黄业昌, 李梦迪, 谢尚耿, 叶玲珍, 张国平. 大麦籽粒β-葡聚糖含量的全基因组关联分析[J]. 作物学报, 2021, 47(7): 1205-1214.
[15] 马娟, 曹言勇, 李会勇. 玉米穗轴粗全基因组关联分析[J]. 作物学报, 2021, 47(7): 1228-1238.
Viewed
Full text


Abstract

Cited

  Shared   
  Discussed   
No Suggested Reading articles found!