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作物学报 ›› 2017, Vol. 43 ›› Issue (11): 1622-1631.doi: 10.3724/SP.J.1006.2017.01622

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

利用分子标记辅助选择聚合水稻Pi-taPi-bWx-mq基因

姚姝,陈涛,张亚东,朱镇,赵庆勇,周丽慧,赵凌,赵春芳,王才林   

  1. 江苏省农业科学院粮食作物研究所 / 江苏省优质水稻工程技术研究中心 / 国家水稻改良中心南京分中心,江苏南京 210014
  • 收稿日期:2017-03-03 修回日期:2017-07-23 出版日期:2017-11-12 网络出版日期:2017-08-02
  • 通讯作者: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307
  • 基金资助:

    本研究由江苏省农业科技自主创新基金项目(CX[12]1003), 江苏省重点研发计划项目(BE2016370)和国家现代农业产业技术体系建设专项 (CARS-01-47)资助。

Pyramiding Pi-ta, Pi-b and Wx-mq Genes by Marker-assisted Selection in Rice (Oryza sativa L.)

YAO Shu, CHEN Tao,ZHANG Ya-Dong,ZHU Zhen,ZHAO Qing-Yong,ZHOU Li-Hui,ZHAO Ling,ZHAO Chun-Fang,WANG Cai-Lin*   

  1. Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R&D Center,/Nanjing Branch of China National Center for Rice Improvement, Nanjing 210014, China
  • Received:2017-03-03 Revised:2017-07-23 Published:2017-11-12 Published online:2017-08-02
  • Contact: 王才林, E-mail: clwang@jaas.ac.cn, Tel: 025-84390307
  • Supported by:

    This study was supported by the Agricultural Science and Technology Independent Innovation Fund of Jiangsu (CX [12]1003), the Key Research and Development Projects of Jiangsu Province (BE2016370), and the Special Funds for the Construction of Modern Agricultural Industrial Technology System (CARS-01-47).

摘要:

近年来,优良食味粳稻品种南粳46、南粳5055和南粳9108在江苏等地大面积推广,促进了优质稻米产业的发展。但这些品种均不抗稻瘟病,且缺乏适合在淮北地区种植的中熟中粳型优良食味粳稻品种。本研究以同时携带稻瘟病抗性基因Pi-ta和Pi-b的江苏抗病、高产粳稻品种武粳15为母本,携带低直链淀粉含量基因Wx-mq的优良食味粳稻品种南粳5055为父本配置杂交组合进行聚合育种。利用Pi-ta和Pi-b基因的分子标记多重PCR体系以及Wx-mq基因的四引物扩增受阻突变体系PCR检测技术,分别在不同的分离世代对目标基因位点进行检测,结合田间多代选育、抗性鉴定和籽粒胚乳外观鉴定,成功地将Pi-ta、Pi-b和Wx-mq基因聚合于一体,选育出稻瘟病抗性好、食味品质优、产量高的水稻新品系“南粳0051”,适合在江苏省淮北地区种植。本研究将三套自主研发的PCR检测体系成功应用于分子标记辅助选择,不仅为水稻多基因聚合育种提供了快捷、高效的选择方法,也为水稻抗病、优质育种创制了新的种质资源。

关键词: 水稻, 稻瘟病, 低直链淀粉含量, Pi-ta, Pi-b, Wx-mq, 分子标记辅助选择

Abstract:

In recent years, japonica rice variety Nanjing 46, Nanjing 5055 and Nanjing 9108 were planted in a large area in Jiangsu province because of their good eating quality. However, these varieties are not resistant to rice blast, and there is no japonica rice variety with good eating quality and suitable for planting in Huaibei area, Jiangsu province. The goal of modern rice breeding is to develop new varieties with good quality, high yield and multi-resistance. Marker-assisted selection combined with traditional breeding methods can greatly improve breeding efficiency. In this study, the high yield rice variety “Wujing 15” contained two blast resistance genes of Pi-ta and Pi-b was used as gene recipient to be crossed with the rice variety “Nanjing 5055”, which carries the low-amylose content gene Wx-mq. Self-designed multiple PCR system to detect rice blast resistance genes of Pi-ta and Pi-b and Tetra-primer Amplification Refractory Mutation PCR System for Wx-mq were used to detect the target loci in separation generation. Combining with many generations of breeding in the field, resistance identification, and classification of the grain endosperm appearance, a new japonica rice line “Nanjing 0051” with excellent agronomic traits and suitable for planting in Huaibei area, Jiangsu province was bred by multi-generational selection on resistance and endosperm appearance. This study successfully used the three sets of PCR detection system in the marker-assisted selection, providing a rapid selection method in rice breeding also important intermediate materials for quality and disease-resistance.

Key words: Rice, Blast, Low-amylose content, Pi-ta, Pi-b, Wx-mq, Marker-assisted selection

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