欢迎访问作物学报，今天是
作物学报

作物学报 ›› 2020, Vol. 46 ›› Issue (7): 1006-1015.doi: 10.3724/SP.J.1006.2020.93048

• 作物遗传育种·种质资源·分子遗传学 • 上一篇    下一篇

兼容型maizeSNP384标记筛选与玉米杂交种DNA指纹图谱构建

田红丽**,杨扬**,王璐,王蕊,易红梅,许理文,张云龙,葛建镕,王凤格(),赵久然()   

  1. 北京市农林科学院玉米研究中心/玉米DNA指纹及分子育种北京市重点实验室, 北京100097
  • 收稿日期:2019-08-30 接受日期:2020-03-24 出版日期:2020-07-12 网络出版日期:2020-04-17
  • 通讯作者: 王凤格,赵久然
  • 作者简介:田红丽, E-mail: tianhongli9963@163.com|杨扬, E-mail: caurwx@163.com
    ** 同等贡献
  • 基金资助:
    “十三五”国家重点研发计划项目(2017YFD0102001-003);北京学者计划项目(BSP041)

Screening of compatible maizeSNP384 markers and the construction of DNA fingerprints of maize varieties

TIAN Hong-Li**,YANG Yang**,WANG Lu,WANG Rui,YI Hong-Mei,XU Li-Wen,ZHANG Yun-Long,GE Jian-Rong,WANG Feng-Ge(),ZHAO Jiu-Ran()   

  1. Maize Research Center, Beijing Academy of Agriculture and Forestry Sciences / Beijing Key Laboratory of Maize DNA Fingerprinting and Molecular Breeding, Beijing 100097, China
  • Received:2019-08-30 Accepted:2020-03-24 Published:2020-07-12 Published online:2020-04-17
  • Contact: Feng-Ge WANG,Jiu-Ran ZHAO
  • About author:** Contributed equally to this work
  • Supported by:
    13th Five-Year National Key Research and Development Program of China(2017YFD0102001-003);Beijing Scholars Program(BSP041)

RichHTML

11

PDF

184

摘要：

为加强玉米品种管理和知识产权保护, 评估确定了一套兼容多技术平台, 适于玉米分子鉴定的SNP位点组合, 该组合包含384个位点; 构建了335个玉米杂交种SNP-DNA指纹, 并针对位点和样品从各个角度进行分析。384个位点全部分布在基因内区域, 显示了较好的多态性; MAF、PIC、DP平均值分别为0.39、0.36、0.60, 384个位点中88%的位点MAF值高于0.30, 98%的位点PIC值高于0.30, 98%的位点DP值高于0.50。对335个玉米杂交种进行遗传相似系数两两比较, GD (1 - Nei遗传距离)的变异范围为0.60~0.99, GD≥0.98、0.95、0.90者分别占比0.10%、0.38%、1.40%; GS (相同等位基因比)的变异范围为0.50~0.99, GS≥0.98、0.95、0.90者分别占比0.03%、0.11%、0.35%。从384个位点中抽取最优位点组合, 12个位点组合时品种识别率为0.99, 20个位点组合能够识别335个品种。综上所述, 本研究报道的384个核心SNP位点具有兼容多平台、高稳定性、高重复性、高品种区分能力, 利用核心位点构建了335个玉米杂交种SNP-DNA指纹, 为玉米品种分子鉴定、指纹数据构建以及分子育种提供了关键数据支撑。

关键词: 玉米, SNP, 位点组合, 品种分子鉴定, DNA指纹图谱, 芯片

Abstract:

In order to improve the management of maize varieties and the protection of intellectual property rights, a compatible multiple platform loci combination maizeSNP384 was developed as a means of identifying varieties of maize. SNP-DNA fingerprints of 335 maize hybrids were constructed. All the 384 SNPs were located in the intra-genic region, showing good polymorphism. The average MAF, PIC, and DP of 384 loci were 0.39, 0.36, and 0.60, respectively. MAF in 88% of the 384 loci was most than 0.30, PIC in 98% was most than 0.30, and DP in 98% was most than 0.50. Pairwise comparative analysis of 335 maize hybrids using genetic similarity showed that the range of variation of GD (1-Nei genetic distance) was 0.60-0.99, and the ratios of GD value greater than or equal to 0.98, 0.95, and 0.90 were 0.10%, 0.38%, and 1.40%, respectively. The range of variation of GS (same allele ratio) was 0.50-0.99, and the ratios of GS value greater than or equal to 0.98, 0.95, and 0.90 were 0.03%, 0.11%, and 0.35%, respectively. The optimal locus combination was extracted from 384 SNPs, with the variety discrimination power of 0.99 when 12 SNPs were extracted; the combination of 20 SNPs was able to identify all 335 varieties. In summary, 384 core SNPs reported in this study are compatible with multiple platforms, highly stable, highly repeatable and having high variety discrimination power. SNP-DNA fingerprints of 335 maize hybrids were constructed based on these core SNPs, which provides key data support for molecular identification, fingerprint data construction, and molecular breeding of maize.

Key words: maize, SNP, loci combination, variety molecular identification, DNA fingerprint, chip

表1

用于评估SNP位点的30套杂交种及其双亲的样品信息"

编号
No.		 品种
Variety		 母本
Female parent		 父本
Male parent
T01 SC704 B73 Mo17
T02 丹玉39 Danyu 39 C8605-2 丹598 Dan 598
T03 登海11 Denghai 11 DH65232 DH40
T04 登海9号 Denghai 9 DH65232 8723
T05 东单60 Dongdan 60 A801 丹598 Dan 598
T06 会单4号 Huidan 4 SW1611 掖107 Ye 107
T07 吉单180 Jidan 180 吉853 Ji 853 Mo17
T08 吉单209 Jidan 209 8902 吉853 Ji 853
T09 京科968 Jingke 968 京724 Jing 724 京92 Jing 92
T10 京科糯2000 Jingkenuo 2000 京糯6 Jingnuo 6 白糯6 Bainuo 6
T11 浚单20 Xundan 20 浚9058 Xun 9058 浚92-8 Xun 92-8
T12 蠡玉6号 Liyu 6 543 连87 Lian 87
T13 龙单13 Longdan 13 K10 龙抗11 Longkang 11
T14 鲁单981 Ludan 981 齐319 Qi 319 Lx 9801
T15 农大108 Nongda 108 X178 黄C Huang C
T16 农大3138 Nongda 3138 综31 Zong 31 P138
T17 农华101 Nonghua 101 NH60 S121
T18 沈单10 Shendan 10 沈137 Shen 137 Q1261
T19 沈单16 Shendan 16 K12 沈137 Shen 137
T20 四单19 Sidan 19 444 Mo17
T21 苏玉糯1号 Suyunuo 1 通系5号 Tongxi 5 衡白522 Hengbai 522
T22 苏玉糯5号 Suyunuo 5 通系5号 Tongxi 5 通366 Tong 366
T23 先玉335 Xianyu 335 PH6WC PH4CV
T24 掖单13 Yedan 13 掖478 Ye 478 丹340 Dan 340
T25 渝单8号 Yudan 8 掖478 Ye 478 渝561 Yu 561
T26 渝糯7号 Yunuo 7 S147 S181
T27 豫玉22 Yuyu 22 综3 Zong 3 豫自87-1 Yuzi 87-1
T28 正大619 Zhengda 619 F06 F19
T29 郑单958 Zhengdan 958 郑58 Zheng 58 昌7-2 Chang 7-2
T30 中单2号 Zhongdan 2 Mo17 自330 Zi 330

图1

SNP位点(MG004)在3种芯片平台上的分型图 A: illumina GGGT芯片平台; B: illumina infinium芯片平台; C: affymetrix-axiom芯片平台。图中每个点为1个基因型数据, 相同基因型的数据点聚集在一起; 两侧红色和蓝色的2组数据点为纯合基因型, 中间1组数据点为杂合基因型。"

附表1

适于玉米分子鉴定的384个SNP位点信息,物理位置参考B73参考基因组AGP-V3版本"

位点
Locus		 染色体
Chr.		 物理位置
Physical location		 等位基因A Allele A 等位基因B Allele B MAF DP PIC AB Rate
MG001 1 2304391 A G 0.49 0.62 0.38 0.51
MG002 1 8974336 A G 0.50 0.62 0.38 0.51
MG003 1 11486897 A G 0.43 0.63 0.37 0.47
MG004 1 17738926 G A 0.41 0.60 0.37 0.53
MG005 1 21466180 C T 0.33 0.60 0.35 0.42
MG006 1 24584752 T G 0.43 0.61 0.37 0.51
MG007 1 27804659 C T 0.39 0.61 0.36 0.47
MG008 1 32232188 G A 0.33 0.60 0.35 0.43
MG009 1 35047741 A G 0.43 0.64 0.37 0.44
MG010 1 39118219 A G 0.32 0.59 0.34 0.42
MG011 1 42927856 T C 0.25 0.54 0.31 0.37
MG012 1 44545185 C A 0.36 0.56 0.36 0.55
MG013 1 51014726 G A 0.47 0.57 0.37 0.58
MG014 1 56566037 C A 0.30 0.58 0.33 0.42
MG015 1 61632511 T C 0.45 0.60 0.37 0.54
MG016 1 67735307 C T 0.50 0.57 0.38 0.59
MG017 1 77806149 C T 0.25 0.54 0.31 0.38
MG018 1 79425465 C T 0.46 0.64 0.37 0.47
MG019 1 92354027 G A 0.35 0.58 0.35 0.49
MG020 1 97688251 C A 0.42 0.62 0.37 0.49
MG021 1 111288396 G A 0.42 0.63 0.37 0.47
MG022 1 121979974 T G 0.38 0.60 0.36 0.49
MG023 1 155443646 G A 0.33 0.58 0.35 0.47
MG024 1 156554418 G T 0.38 0.60 0.36 0.49
MG025 1 161723857 C T 0.32 0.58 0.34 0.47
MG026 1 162755217 A G 0.35 0.59 0.35 0.48
MG027 1 164572994 T C 0.18 0.45 0.25 0.26
MG028 1 171553774 C T 0.34 0.57 0.35 0.51
MG029 1 180966708 T G 0.32 0.57 0.34 0.47
MG030 1 198287528 A G 0.46 0.65 0.37 0.44
MG031 1 200899746 T C 0.45 0.62 0.37 0.51
MG032 1 209627876 G A 0.32 0.59 0.34 0.43
MG033 1 220359715 C T 0.33 0.60 0.34 0.43
MG034 1 243187260 G C 0.30 0.58 0.33 0.43
MG035 1 245753063 A G 0.48 0.59 0.37 0.56
MG036 1 248798195 T C 0.25 0.54 0.31 0.36
MG037 1 253565722 C T 0.30 0.58 0.33 0.43
MG038 1 263219431 G A 0.35 0.60 0.35 0.44
MG039 1 265515707 A C 0.42 0.63 0.37 0.45
MG040 1 269419877 T G 0.26 0.55 0.32 0.41
MG041 1 272938380 A G 0.31 0.56 0.34 0.49
MG042 1 276881002 C T 0.36 0.60 0.35 0.46
MG043 1 277668429 G A 0.41 0.61 0.37 0.49
MG044 1 279336197 T C 0.41 0.62 0.37 0.48
MG045 1 281272643 G A 0.41 0.60 0.37 0.51
MG046 1 282748902 T C 0.45 0.58 0.37 0.56
MG047 1 285846709 C T 0.35 0.61 0.35 0.43
MG048 1 287789543 T C 0.36 0.61 0.35 0.42
MG049 1 289408285 T C 0.46 0.58 0.37 0.57
MG050 1 290379030 T C 0.49 0.64 0.38 0.48
MG051 1 292481023 T C 0.46 0.64 0.37 0.47
MG052 1 296850785 C T 0.33 0.59 0.35 0.46
MG053 1 298863606 C T 0.49 0.62 0.38 0.51
MG054 2 2500805 C T 0.33 0.58 0.35 0.48
MG055 2 3743906 A G 0.49 0.60 0.40 0.55
MG056 2 4697714 C A 0.31 0.56 0.34 0.49
MG057 2 6915357 A G 0.36 0.56 0.36 0.54
MG058 2 8914166 T C 0.46 0.61 0.37 0.53
MG059 2 22258930 G A 0.36 0.57 0.36 0.53
MG060 2 34162472 G A 0.36 0.58 0.35 0.51
MG061 2 47154261 A G 0.40 0.61 0.37 0.50
MG062 2 47789173 T C 0.35 0.60 0.35 0.47
MG063 2 57693847 G A 0.28 0.55 0.32 0.46
MG064 2 109563976 G T 0.34 0.54 0.35 0.56
MG065 2 111835040 C A 0.45 0.65 0.37 0.43
MG066 2 113226924 A C 0.45 0.55 0.37 0.61
MG067 2 145265362 T C 0.47 0.57 0.37 0.59
MG068 2 152240139 G T 0.27 0.56 0.32 0.35
MG069 2 156515220 A C 0.45 0.55 0.37 0.60
MG070 2 160621095 C T 0.47 0.56 0.37 0.60
MG071 2 182623845 G A 0.49 0.57 0.38 0.59
MG072 2 186339948 T G 0.47 0.60 0.37 0.54
MG073 2 191081573 C T 0.48 0.57 0.38 0.59
MG074 2 195937060 C A 0.45 0.62 0.37 0.50
MG075 2 196568843 T G 0.34 0.59 0.35 0.45
MG076 2 199837334 C T 0.27 0.56 0.32 0.40
MG077 2 201108308 C A 0.42 0.61 0.37 0.50
MG078 2 204601534 C T 0.37 0.60 0.36 0.49
MG079 2 207814715 A G 0.37 0.63 0.36 0.37
MG080 2 213018299 G A 0.39 0.61 0.36 0.48
MG081 2 216183437 T C 0.25 0.53 0.30 0.41
MG082 2 219129445 T C 0.27 0.54 0.32 0.44
MG083 2 221291383 C A 0.31 0.59 0.34 0.39
MG084 2 223977328 T G 0.42 0.62 0.37 0.48
MG085 2 225264554 T G 0.25 0.54 0.30 0.38
MG086 2 226199968 A G 0.40 0.61 0.37 0.49
MG087 2 235077065 C T 0.45 0.59 0.37 0.55
MG088 3 8979286 C T 0.37 0.58 0.36 0.51
MG089 3 15222625 G T 0.37 0.62 0.36 0.41
MG090 3 24225424 A G 0.34 0.57 0.35 0.50
MG091 3 25866687 G A 0.30 0.58 0.33 0.39
MG092 3 27775731 T C 0.47 0.54 0.37 0.63
MG093 3 29213820 T G 0.46 0.62 0.37 0.51
MG094 3 54969279 G T 0.49 0.59 0.38 0.56
MG095 3 57091132 C A 0.31 0.58 0.34 0.44
MG096 3 58082419 A G 0.30 0.56 0.33 0.47
MG097 3 67702516 G A 0.43 0.63 0.37 0.47
MG098 3 92555979 T C 0.36 0.59 0.35 0.50
MG099 3 88400489 C T 0.46 0.63 0.37 0.48
MG100 3 117106253 C A 0.37 0.61 0.36 0.46
MG101 3 118083714 C A 0.49 0.51 0.38 0.66
MG102 3 122901197 C T 0.49 0.61 0.38 0.52
MG103 3 126128781 C T 0.44 0.57 0.37 0.57
MG104 3 132489439 C T 0.46 0.62 0.37 0.51
MG105 3 134796979 A C 0.45 0.60 0.37 0.54
MG106 3 141646415 C T 0.50 0.63 0.38 0.50
MG107 3 142724956 T C 0.30 0.55 0.33 0.48
MG108 3 151086172 T C 0.40 0.61 0.36 0.50
MG109 3 152533264 A G 0.36 0.59 0.35 0.48
MG110 3 155358367 G A 0.34 0.61 0.35 0.42
MG111 3 156325759 A G 0.41 0.59 0.37 0.53
MG112 3 161301281 A G 0.48 0.63 0.38 0.49
MG113 3 162340416 G A 0.39 0.62 0.36 0.46
MG114 3 169261670 A G 0.48 0.65 0.38 0.45
MG115 3 173048348 T C 0.40 0.63 0.37 0.45
MG116 3 174581037 T C 0.33 0.58 0.35 0.48
MG117 3 179842209 A G 0.49 0.61 0.38 0.53
MG118 3 181032542 T C 0.34 0.60 0.35 0.43
MG119 3 184176645 T G 0.36 0.59 0.36 0.50
MG120 3 196000794 C T 0.44 0.62 0.37 0.49
MG121 3 200262266 T C 0.38 0.62 0.36 0.45
MG122 3 201757644 G A 0.49 0.60 0.38 0.54
MG123 3 203824251 A C 0.42 0.61 0.37 0.51
MG124 3 206611097 G A 0.30 0.58 0.33 0.37
MG125 3 208142658 T C 0.35 0.61 0.35 0.41
MG126 3 209109183 T G 0.30 0.59 0.33 0.38
MG127 3 212050055 A C 0.46 0.57 0.37 0.58
MG128 3 218112568 T C 0.44 0.62 0.37 0.50
MG129 3 221653490 A C 0.26 0.55 0.31 0.34
MG130 3 226343075 A G 0.46 0.62 0.37 0.50
MG131 3 229397149 T G 0.38 0.63 0.36 0.41
MG132 4 1487851 G A 0.26 0.55 0.31 0.37
MG133 4 14131426 A G 0.42 0.58 0.37 0.56
MG134 4 18832691 A C 0.41 0.60 0.37 0.51
MG135 4 20077010 G T 0.39 0.59 0.36 0.51
MG136 4 20761638 A C 0.21 0.49 0.27 0.31
MG137 4 22576276 T C 0.28 0.56 0.32 0.41
MG138 4 31743151 T G 0.30 0.58 0.33 0.40
MG139 4 33150343 T C 0.45 0.65 0.37 0.40
MG140 4 37381509 A T 0.33 0.61 0.34 0.37
MG141 4 39828005 T C 0.39 0.62 0.36 0.46
MG142 4 45151079 C T 0.47 0.60 0.37 0.55
MG143 4 50184084 T G 0.35 0.57 0.35 0.52
MG144 4 53473032 A G 0.41 0.60 0.37 0.51
MG145 4 55889650 C T 0.49 0.64 0.38 0.47
MG146 4 67519590 T C 0.48 0.63 0.37 0.49
MG147 4 77234543 A G 0.49 0.62 0.38 0.52
MG148 4 128874466 G T 0.33 0.58 0.35 0.47
MG149 4 145650338 A G 0.35 0.58 0.35 0.51
MG150 4 149311096 G A 0.39 0.64 0.36 0.35
MG151 4 151253200 A G 0.37 0.62 0.36 0.44
MG152 4 153661937 G A 0.44 0.64 0.37 0.45
MG153 4 154880065 C T 0.41 0.61 0.37 0.51
MG154 4 164294138 C T 0.30 0.56 0.33 0.47
MG155 4 169994302 G A 0.49 0.63 0.38 0.49
MG156 4 173238441 A G 0.31 0.59 0.34 0.39
MG157 4 177180130 A G 0.46 0.58 0.37 0.57
MG158 4 179824742 C T 0.48 0.61 0.38 0.53
MG159 4 184328972 G A 0.26 0.55 0.31 0.38
MG160 4 193937034 T C 0.39 0.59 0.36 0.52
MG161 4 201638107 A G 0.41 0.62 0.37 0.47
MG162 4 208661194 T C 0.32 0.58 0.34 0.46
MG163 4 215441456 A G 0.32 0.58 0.34 0.45
MG164 4 221839256 G T 0.34 0.61 0.35 0.40
MG165 4 223648151 G A 0.45 0.61 0.37 0.51
MG166 4 230935341 T G 0.40 0.58 0.37 0.55
MG167 4 232019010 A C 0.40 0.59 0.36 0.52
MG168 4 234081042 G T 0.31 0.56 0.34 0.50
MG169 4 236715495 C T 0.42 0.62 0.37 0.49
MG170 4 238918388 G A 0.35 0.61 0.35 0.43
MG171 4 240891845 T G 0.39 0.58 0.36 0.53
MG172 5 6881507 A G 0.40 0.63 0.37 0.45
MG173 5 8507286 C T 0.33 0.60 0.34 0.40
MG174 5 11356449 T C 0.32 0.56 0.34 0.51
MG175 5 13402375 T C 0.45 0.60 0.37 0.54
MG176 5 15452020 C T 0.27 0.55 0.32 0.43
MG177 5 17881372 G A 0.49 0.64 0.38 0.47
MG178 5 21008784 T C 0.39 0.61 0.36 0.47
MG179 5 29937148 C T 0.42 0.63 0.37 0.44
MG180 5 31668063 C T 0.48 0.65 0.37 0.44
MG181 5 33072014 G A 0.43 0.58 0.37 0.56
MG182 5 35669253 G A 0.29 0.57 0.33 0.40
MG183 5 38831562 T C 0.17 0.44 0.25 0.24
MG184 5 48413218 G T 0.47 0.63 0.37 0.50
MG185 5 59068812 T C 0.48 0.63 0.38 0.48
MG186 5 60955626 C T 0.37 0.59 0.36 0.51
MG187 5 62137456 A G 0.26 0.54 0.31 0.40
MG188 5 64134193 G A 0.33 0.57 0.34 0.49
MG189 5 72721863 C T 0.31 0.58 0.33 0.41
MG190 5 74941891 A G 0.27 0.56 0.32 0.39
MG191 5 80839340 G A 0.45 0.60 0.37 0.54
MG192 5 86070543 C T 0.33 0.57 0.34 0.49
MG193 5 88226330 G A 0.40 0.61 0.37 0.48
MG194 5 93618116 A G 0.30 0.57 0.33 0.43
MG195 5 130860915 T C 0.48 0.62 0.37 0.51
MG196 5 141206885 C T 0.44 0.65 0.37 0.42
MG197 5 161040798 G T 0.30 0.58 0.33 0.39
MG198 5 163451299 C T 0.37 0.63 0.36 0.38
MG199 5 166437548 T C 0.38 0.63 0.36 0.40
MG200 5 173209693 G A 0.38 0.62 0.36 0.44
MG201 5 174352063 A G 0.46 0.63 0.37 0.49
MG202 5 176085344 G A 0.43 0.59 0.37 0.55
MG203 5 178878084 T C 0.42 0.64 0.37 0.42
MG204 5 181267545 A G 0.36 0.60 0.36 0.48
MG205 5 190533458 G A 0.48 0.64 0.38 0.47
MG206 5 199812574 T C 0.49 0.61 0.38 0.53
MG207 5 204879476 G A 0.39 0.58 0.36 0.54
MG208 5 215521126 T C 0.35 0.62 0.35 0.39
MG209 5 217664096 C T 0.49 0.62 0.38 0.51
MG210 6 3592433 A G 0.45 0.61 0.37 0.52
MG211 6 4843861 T C 0.40 0.63 0.37 0.43
MG212 6 6530602 G A 0.38 0.61 0.36 0.47
MG213 6 8111698 A C 0.35 0.59 0.35 0.49
MG214 6 33247659 G A 0.48 0.64 0.37 0.48
MG215 6 39822979 C T 0.50 0.55 0.38 0.62
MG216 6 42757005 T G 0.48 0.60 0.38 0.54
MG217 6 45872006 G T 0.37 0.60 0.36 0.49
MG218 6 53883513 T G 0.27 0.56 0.32 0.36
MG219 6 56274915 G A 0.25 0.53 0.30 0.42
MG220 6 56968490 C G 0.36 0.60 0.36 0.47
MG221 6 61133030 G T 0.49 0.62 0.40 0.53
MG222 6 80804393 C T 0.37 0.58 0.36 0.52
MG223 6 83130638 G A 0.50 0.64 0.38 0.47
MG224 6 86421059 T A 0.32 0.57 0.34 0.49
MG225 6 93160349 A G 0.40 0.59 0.37 0.53
MG226 6 110465918 C T 0.50 0.66 0.38 0.41
MG227 6 111230603 C T 0.40 0.61 0.37 0.49
MG228 6 108233804 A T 0.37 0.62 0.36 0.43
MG229 6 110053930 G A 0.49 0.63 0.38 0.49
MG230 6 114361855 C T 0.47 0.59 0.37 0.55
MG231 6 118325927 C A 0.44 0.61 0.37 0.51
MG232 6 120823677 A G 0.33 0.60 0.34 0.39
MG233 6 123789176 C T 0.36 0.59 0.35 0.48
MG234 6 125370735 G A 0.42 0.58 0.37 0.56
MG235 6 132222944 T C 0.31 0.57 0.34 0.45
MG236 6 133537579 C T 0.48 0.62 0.37 0.51
MG237 6 134282494 C T 0.38 0.61 0.36 0.48
MG238 6 136548390 T C 0.34 0.59 0.35 0.45
MG239 6 139059651 T C 0.21 0.50 0.28 0.38
MG240 6 141476300 T G 0.43 0.61 0.37 0.52
MG241 6 144146341 G A 0.34 0.59 0.35 0.46
MG242 6 151680442 T C 0.37 0.61 0.36 0.45
MG243 6 153168568 C T 0.30 0.56 0.33 0.48
MG244 6 154359722 A G 0.37 0.62 0.36 0.44
MG245 6 158855833 A G 0.47 0.62 0.37 0.50
MG246 6 161650242 G A 0.49 0.62 0.38 0.51
MG247 6 164878483 T C 0.35 0.62 0.35 0.40
MG248 7 1196275 T C 0.33 0.58 0.35 0.49
MG249 7 5558292 T C 0.49 0.63 0.38 0.49
MG250 7 8069959 G A 0.41 0.64 0.37 0.42
MG251 7 9336550 A G 0.35 0.61 0.35 0.42
MG252 7 13214476 C A 0.34 0.59 0.35 0.47
MG253 7 20905062 C T 0.47 0.61 0.37 0.53
MG254 7 40598911 T C 0.25 0.54 0.30 0.36
MG255 7 44152949 A G 0.43 0.63 0.37 0.46
MG256 7 50103439 T C 0.46 0.62 0.37 0.51
MG257 7 54347237 G A 0.36 0.60 0.35 0.47
MG258 7 82347040 G A 0.30 0.57 0.33 0.43
MG259 7 86087257 A G 0.34 0.59 0.35 0.48
MG260 7 88150664 T G 0.36 0.62 0.36 0.40
MG261 7 94053087 T C 0.30 0.57 0.33 0.43
MG262 7 66727257 C A 0.32 0.58 0.34 0.47
MG263 7 98592776 G A 0.43 0.56 0.37 0.58
MG264 7 101605140 A C 0.35 0.60 0.35 0.46
MG265 7 106782474 C T 0.45 0.62 0.37 0.49
MG266 7 109082463 T G 0.45 0.64 0.37 0.46
MG267 7 117397385 T G 0.37 0.59 0.36 0.51
MG268 7 120009353 G A 0.35 0.58 0.35 0.49
MG269 7 123883434 G A 0.46 0.64 0.37 0.45
MG270 7 126677146 C A 0.33 0.58 0.34 0.47
MG271 7 132197633 A G 0.36 0.57 0.35 0.52
MG272 7 133597448 C T 0.36 0.59 0.36 0.49
MG273 7 136307948 T C 0.38 0.62 0.36 0.44
MG274 7 137524243 G A 0.32 0.59 0.34 0.40
MG275 7 143446474 G A 0.39 0.62 0.36 0.45
MG276 7 144541791 G A 0.29 0.57 0.33 0.41
MG277 7 148219064 A G 0.38 0.63 0.36 0.43
MG278 7 153138112 A G 0.43 0.62 0.37 0.50
MG279 7 155821714 A G 0.42 0.61 0.37 0.50
MG280 7 157609773 A C 0.35 0.62 0.35 0.34
MG281 7 158991658 C T 0.45 0.65 0.37 0.42
MG282 7 162196275 A G 0.37 0.57 0.36 0.54
MG283 7 163637365 C T 0.47 0.63 0.37 0.48
MG284 7 169245453 C T 0.48 0.64 0.37 0.47
MG285 7 172200651 T C 0.36 0.57 0.36 0.53
MG286 7 175252105 G A 0.43 0.65 0.37 0.38
MG287 8 2761824 G A 0.43 0.62 0.37 0.48
MG288 8 4177172 G T 0.48 0.59 0.38 0.56
MG289 8 7624059 T G 0.28 0.56 0.32 0.43
MG290 8 10124026 T C 0.48 0.63 0.38 0.49
MG291 8 14102678 C T 0.39 0.62 0.36 0.44
MG292 8 15996125 G A 0.39 0.60 0.36 0.50
MG293 8 20978845 T G 0.45 0.61 0.37 0.52
MG294 8 26588701 G A 0.34 0.58 0.35 0.48
MG295 8 40391144 A G 0.39 0.63 0.36 0.44
MG296 8 59490912 G A 0.37 0.63 0.36 0.39
MG297 8 65658110 A C 0.29 0.55 0.33 0.47
MG298 8 67244579 G A 0.49 0.60 0.38 0.55
MG299 8 70314230 G A 0.31 0.57 0.34 0.45
MG300 8 73372505 C A 0.32 0.60 0.34 0.40
MG301 8 77266692 C T 0.37 0.62 0.36 0.42
MG302 8 78372314 A G 0.45 0.58 0.37 0.57
MG303 8 84128111 T C 0.31 0.57 0.34 0.45
MG304 8 85385460 T C 0.44 0.60 0.37 0.54
MG305 8 97932001 G A 0.34 0.59 0.35 0.46
MG306 8 100450253 A G 0.23 0.52 0.29 0.35
MG307 8 109898576 C G 0.20 0.48 0.27 0.31
MG308 8 111252477 A G 0.30 0.58 0.33 0.43
MG309 8 113184750 T C 0.42 0.65 0.37 0.41
MG310 8 117143671 A G 0.40 0.62 0.36 0.47
MG311 8 118299376 C T 0.36 0.54 0.35 0.57
MG312 8 124844061 A G 0.42 0.62 0.37 0.49
MG313 8 126875542 T C 0.31 0.58 0.34 0.43
MG314 8 137758805 G A 0.33 0.59 0.34 0.45
MG315 8 139128429 C T 0.35 0.59 0.35 0.48
MG316 8 144758077 T C 0.35 0.59 0.35 0.49
MG317 8 146150639 G T 0.24 0.52 0.30 0.42
MG318 8 147803381 T C 0.43 0.65 0.39 0.44
MG319 8 152624007 G A 0.37 0.58 0.36 0.51
MG320 8 153398996 C T 0.30 0.56 0.33 0.47
MG321 8 158931728 T C 0.31 0.58 0.34 0.43
MG322 8 161316216 C A 0.30 0.58 0.33 0.42
MG323 8 164483853 A G 0.39 0.62 0.36 0.46
MG324 8 165927214 G T 0.48 0.64 0.38 0.46
MG325 8 170942747 A G 0.41 0.62 0.37 0.48
MG326 8 172618390 T C 0.48 0.65 0.37 0.45
MG327 8 174329510 G A 0.32 0.59 0.34 0.42
MG328 9 1025355 G A 0.44 0.57 0.37 0.59
MG329 9 3857368 T C 0.44 0.59 0.37 0.54
MG330 9 7191207 A G 0.38 0.59 0.36 0.51
MG331 9 11505100 C T 0.47 0.61 0.37 0.53
MG332 9 12582466 G A 0.34 0.59 0.35 0.46
MG333 9 15999332 A C 0.30 0.57 0.33 0.44
MG334 9 17446904 G T 0.41 0.62 0.37 0.47
MG335 9 24162919 T A 0.33 0.56 0.34 0.52
MG336 9 27238881 A G 0.33 0.57 0.35 0.49
MG337 6 117204743 A G 0.47 0.64 0.37 0.45
MG338 9 48613774 T C 0.39 0.64 0.36 0.37
MG339 9 56522105 C T 0.36 0.56 0.35 0.54
MG340 9 61772043 C T 0.37 0.63 0.36 0.32
MG341 9 65041502 A G 0.43 0.61 0.37 0.52
MG342 9 68085408 T G 0.32 0.60 0.34 0.37
MG343 9 89991829 G A 0.39 0.63 0.36 0.44
MG344 9 96747891 T C 0.50 0.63 0.38 0.48
MG345 9 98400029 G T 0.46 0.63 0.37 0.48
MG346 9 102277066 T C 0.46 0.61 0.37 0.53
MG347 9 104695670 A G 0.42 0.55 0.37 0.60
MG348 9 124089293 G A 0.33 0.60 0.34 0.43
MG349 9 127197714 A G 0.50 0.55 0.38 0.62
MG350 9 143433554 C T 0.46 0.62 0.37 0.51
MG351 9 148627045 C T 0.49 0.56 0.38 0.60
MG352 9 149308774 A C 0.49 0.65 0.38 0.42
MG353 9 150911119 G A 0.45 0.58 0.37 0.56
MG354 9 151542506 A G 0.49 0.61 0.38 0.53
MG355 9 155054804 G A 0.32 0.57 0.34 0.48
MG356 10 2802838 T G 0.43 0.62 0.37 0.48
MG357 10 10674490 C T 0.21 0.49 0.27 0.35
MG358 10 22133988 C T 0.30 0.57 0.33 0.43
MG359 10 32194186 T C 0.30 0.56 0.33 0.47
MG360 10 58207909 T C 0.45 0.64 0.37 0.45
MG361 10 39960289 C T 0.48 0.59 0.40 0.57
MG362 10 61011906 G A 0.49 0.65 0.38 0.45
MG363 10 76655433 T G 0.50 0.66 0.38 0.40
MG364 10 82039366 G A 0.48 0.61 0.38 0.53
MG365 10 84110544 T G 0.33 0.59 0.35 0.44
MG366 10 89433313 G A 0.36 0.60 0.36 0.46
MG367 10 95929973 A G 0.46 0.59 0.37 0.55
MG368 10 103380111 A G 0.47 0.62 0.37 0.50
MG369 10 109396730 G A 0.49 0.61 0.38 0.53
MG370 10 114217715 G A 0.49 0.64 0.38 0.46
MG371 10 117773469 T G 0.38 0.60 0.36 0.50
MG372 10 121559630 G A 0.50 0.63 0.38 0.50
MG373 10 129486606 A G 0.30 0.58 0.33 0.39
MG374 10 130399835 A G 0.32 0.58 0.34 0.45
MG375 10 131065538 T C 0.43 0.65 0.37 0.42
MG376 10 131912401 T C 0.49 0.65 0.38 0.44
MG377 10 132535283 G A 0.28 0.55 0.32 0.45
MG378 10 133905339 G A 0.28 0.57 0.32 0.36
MG379 10 135351702 C T 0.35 0.61 0.35 0.41
MG380 10 135937058 T C 0.32 0.59 0.34 0.41
MG381 10 137192651 A C 0.42 0.62 0.37 0.48
MG382 10 139565935 A G 0.41 0.59 0.37 0.54
MG383 10 144384643 A C 0.49 0.64 0.38 0.47
MG384 10 147014927 G A 0.29 0.57 0.33 0.41

图2

maizeSNP384位点组合的分布 A: 384个SNP位点在玉米10对染色体上分布图; B: 384个SNP位点在基因上的分布情况。"

图3

384个SNP位点的MAF和PIC多态性指数变化分布图 横坐标为MAF、PIC的区间值, 纵坐标为SNP位点数目。"

图4

335个玉米杂交种SNP-DNA指纹总体展示图 每行代表1个品种, 按照国家审定编号排序; 每列代表1个SNP位点, 按照在基因组上物理位置排序; DNA指纹分别用不同的颜色表示。"

图5

335个玉米品种的杂合基因型频率、遗传差异分析 A: 335个玉米品种的杂合基因型频率分布(杂合基因型的标记数目/384); B: 335个玉米品种两两成对比较差异位点数分布图, 横坐标为成对比较差异位点数目, 纵坐标为成对比较的数目; C: 335个玉米品种两两成对比较遗传相似系数分布图, 横坐标为遗传相似系数百分比, 纵坐标为成对比较的数目。"

图6

基于最优位点组合的品种识别率变化曲线图"

[1] 唐立群, 肖层林, 王伟平. SNP分子标记的研究及其应用进展. 中国农学通报, 2012,28:154-158.
Tang L Q, Xiao C L, Wang W P. Research and application progress of SNP markers. Chin Agric Bull, 2012,28:154-158 (in Chinese with English abstract).
[2] UPOV (International Union for the Protection of New Varieties of Plants). Possible Used of Molecular Markers in the Examination of Distinctness, Uniformity and Stability (DUS). UPOV, Geneva, Switzerland, 2011.
[3] Mammadov J, Chen W, Mingus J, Thompson S, Kumpatla S. Development of versatile gene-based SNP assays in maize (Zea mays L.). Mol Breed, 2012,29:779-790.
[4] 魏中艳, 李慧慧, 李骏, Gamar Y A, 马岩松, 邱丽娟. 应用SNP精准鉴定大豆种质及构建可扫描身份证. 作物学报, 2018,44:315-323.
Wei Z Y, Li H H, Li J, Gamar Y A, Ma Y S, Qiu L J. Accurate identification of varieties by nucleotide polymorphisms and establishment of scannable variety IDs for soybean germplasm. Acta Agron Sin, 2018,44:315-323 (in Chinese with English abstract).
[5] 李志远, 于海龙, 方智远, 杨丽梅, 刘玉梅, 庄木, 吕红豪, 张扬勇. 甘蓝SNP标记开发及主要品种DNA指纹图谱构建. 中国农业科学, 2018,51:2771-2787.
Li Z Y, Yu H L, Fang Z Y, Yang L M, Liu Y M, Zhuang M, Lyu H H, Zhang Y Y. Development of SNP markers in cabbage and construction of DNA fingerprinting of main varieties. Sci Agric Sin, 2018,51:2771-2787 (in Chinese with English abstract).
[6] Jung J K, Park S W, Liu W Y, Kang B C. Discovery of single nucleotide polymorphism in Capsicum and SNP markers for cultivar identification. Euphytica, 2010,175:91-107.
[7] Kuang M, Wei S J, Wang Y Q, Zhou D Y, Ma L, Fang D, Yang W H, Ma Z Y. Development of a core set of SNP markers for the identification of upland cotton cultivars in China. J Integr Agr, 2016,15:964-962.
[8] Chen H D, Xie W B, He H, Yu H H, Chen W, Li J, Yu R B, Yao Y, Zhang W H, He Y Q, Tang X Y, Zhou F S, Deng X W, Zhang Q F. A high-density SNP genotyping array for rice biology and molecular breeding. Mol Plant, 2014,7:541-553.
pmid: 24121292
[9] Winfield M O, Allen A M, Burridge A J, Barker G L A, Benbow H R, Wilkinson P A, Coghill J, Waterfall C, Davassi A, Scopes G, Pirani A, Webster T, Brew F, Bloor C, King J, West C, Griffiths S, King I, Bentley A R, Edwards K J. High-density SNP genotyping array for hexaploid wheat and its secondary and tertiary gene pool. Plant Biotechnol J, 2016,14:1195-1206.
pmid: 26466852
[10] Song Q J, Hyten D L, Jia G F, Quigley C V, Fickus E W, Nelson R L, Cregan P B. Development and evaluation of SoySNP50K, a high-density genotyping array for soybean. PLoS One, 2013,8:e54985.
doi: 10.1371/journal.pone.0054985 pmid: 23372807
[11] Hulse-Kemp A M, Lemm J, Plieske J, Ashrafi H, Buyyarapu R, Fang D D, Frelichowski J, Giband M, Hague S, Hinze L L, Kochan K J, Riggs P K, Scheffler J A, Udall J A, Ulloa M, Wang S S, Zhu Q H, Bag S K, Bhardwaj A, Burke J J, Byers R L, Claverie M, Gore M A, Harker D B, Islam M S, Jenkins J N, Jones D C, Lacape J M, Llewellyn D J, Percy R G, Pepper A E, Poland J A, Rai K M, Sawant S V, Singh S K, Spriggs A, Taylor J M, Wang F, Yourstone S M, Zheng X T, Lawley C T, Ganal M W, Deynze A V, Wilson I W, Stelly D M. Development of a 63K SNP Array for cotton and high-density mapping of intraspecific and interspecific populations of Gossypium spp. Genes Genomes Genet, 2015,5:1187-1209.
[12] Lai J S, Li R Q, Xu X, Jin W W, Xu M L, Zhao H N, Xiang X K, Song W B, Ying K, Zhang M, Jiao Y P, Ni P X, Zhang J G, Li D, Guo X S, Ye K X, Jian M, Wang B, Zheng H S, Liang H Q, Zhang X Q, Wang S C, Chen S J, Li J S, Fu Y, Springer N M, Yang H M, Wang J, Dai J R, Schnable P S, Wang J. Genome-wide patterns of genetic variation among elite maize inbred lines. Nat Genet, 2010,42:1027-1030.
doi: 10.1038/ng.684 pmid: 20972441
[13] Jiao Y P, Zhao H N, Ren L H, Song W B, Zeng B, Guo J J, Wang B B, Liu Z P, Chen J, Li W, Zhang M, Xie S J, Lai J S. Genome-wide genetic changes during modern breeding of maize. Nat Genet, 2012,44:812-817.
doi: 10.1038/ng.2312 pmid: 22660547
[14] Chia J M, Song C, Bradbury P J, Costich D, Leon N D, Doebley J, Elshire R J, Gaut B, Geller L, Glaubitz J C, Gore M, Guill K E, Holland J, Hufford M B, Lai J S, Li M, Liu X, Lu Y L, McCombie R, Nelson R, Poland J, Prasanna B M, Pyhäjärvi T, Rong T Z, Sekhon R S, Sun Q, Tenaillon M I, Tian F, Wang J, Xu X, Zhang Z U, Kaeppler S M, Ross-Ibarra J, McMullen M D, Buckler E S, Zhang G Y, Xu Y B, Ware D. Maize HapMap2 identifies extant variation from a genome in flux. Nat Genet, 2012,44:803-809.
pmid: 22660545
[15] Ganal M W, Durstewitz G, Polley A, Bérard A, Buckler E S, Charcosset A, Clarke J D, Graner E M, Hansen M, Joets J, Paslier M C, McMullen M D, Montalent P, Rose M, Schön C C, Sun Q, Walter H, Martin O C, Falque M. A large maize (Zea mays L.) SNP genotyping array: development and germplasm genotyping and genetic mapping to compare with the B73 reference genome. PLoS One, 2011,6:e28334.
doi: 10.1371/journal.pone.0028334 pmid: 22174790
[16] Cheng X, Ren Y H, Jian Y Q, Guo Z F, Zhang Y, Xie C X, Fu J J, Wang H W, Wang G Y, Xu Y B, Li P, Zou C. Development of a maize 55K SNP array with improved genome coverage for molecular breeding. Mol Breed, 2017,37:20.
doi: 10.1007/s11032-017-0622-z pmid: 28255264
[17] Jones E, Chu W C, Ayele M, Ho J, Bruggeman E, Yourstone K, Rafalski A, Smith O S, McMullen M D, Bezawada C, Warren J, Babayev J, Basu S, Smith S. Development of single nucleotide polymorphism (SNP) markers for use in commercial maize (Zea mays L.). Mol Breed, 2009,24:165-176.
doi: 10.1007/s11032-009-9281-z
[18] Yan J B, Yang X H, Shah T, Sánchez-Villeda H, Li J S, Warburton M, Zhou Y, Crouch J H, Xu Y B. High-throughput SNP genotyping with the GoldenGate assay in maize. Mol Breed, 2010,25:441-451.
doi: 10.1007/s11032-009-9343-2
[19] Tian H L, Wang F G, Zhao J R, Yi H M, Wang L, Wang R, Yang Y, Song W. Development of maizeSNP3072, a high-throughput compatible SNP array, for DNA fingerprinting identification of Chinese maize varieties. Mol Breed, 2015,35:136.
pmid: 26052247
[20] 王凤格, 赵久然, 杨扬. 国家审定玉米品种SSR指纹图谱. 北京: 中国农业科学技术出版社, 2015.
Wang F G, Zhao J R, Yang Y. SSR Fingerprinting Data of National Approval of Maize Cultivars. Beijing: China Agricultural Science and Technology Press, 2015 (in Chinese).
[21] 王凤格, 易红梅, 赵久然, 刘平, 张新明, 田红丽, 堵苑苑. 玉米品种鉴定技术规程SSR标记法. 中华人民共和国农业行业标准, NY/T1432-2014, 2014.
Wang F G, Yi H M, Zhao J R, Liu P, Zhang X M, Tian H L, Du Y Y. Protocol for the Identification of Maize Varieties—SSR Marker Method. Agricultural Industry Standards of the People's Republic of China, NY/T1432-2014, 2014 (in Chinese).
[22] 王凤格, 赵久然, 杨扬. 基于遗传算法的植物品种真实性鉴定位点筛选方法. 中国发明专利, 2013,ZL201310629676. 2.
Wang F G, Zhao J R, Yang Y. The Method of Locus Selecting for Plant Variety Authenticity Identification Based on Genetic Algorithm. Chinese Invention Patent, 2013, ZL201410495417. X (in Chinese).
[23] Tessier C, David J, This P, Boursiquot J M, Charrier A. Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theor Appl Genet, 1999,98:171-177.
doi: 10.1007/s001220051054
[24] 王凤格, 田红丽, 易红梅, 赵涵, 霍永学, 匡猛, 张力科, 吕远大, 丁曼卿, 赵久然. 植物品种DNA指纹鉴定原理及其鉴定方案. 分子植物育种, 2018,16:4756-4766.
Wang F G, Tian H L, Yi H M, Zhao H, Huo Y X, Kuang M, Zhang L K, Lyu Y D, Ding M Q, Zhao J R. Principle and strategy of DNA fingerprint identification of plant variety. Mol Plant Breed, 2018,16:4756-4766 (in Chinese with English abstract).
[25] 赵久然, 王凤格, 田红丽, 易红梅, 王蕊, 葛建镕. 适于农作物品种分子身份鉴别和确权鉴定的检测方法. 中国发明专利, 2017,201710527354.5.
Zhao J R, Wang F G, Tian H L, Yi H M, Wang R, Ge J R. Detection Methods for Identity Distinguish and Intellectual Property Confirmation of Crop Varieties Using Molecular Markers. Chinese Invention Patent, 2017,ZL201710527354.5 (in Chinese).
[26] Rousselle Y, Jones E, Charcosset A, Moreau P, Robbins K, Stich B, Knaak C, Flament P, Karaman Z, Martinant J R, Fourneau M, Taillardat A, Romestant M, Tabel C, Bertran J, Ranc N, Lespinasse D, Blanchard P, Kahler A, Chen J, Kahler J, Dobrin S, Warner T, Ferris R, Smith S. Study on essential derivation in maize: III. Selection and evaluation of a panel of single nucleotide polymorphism loci for use in European and North American Germplasm. Crop Sci, 2015,55:1170-1180.
doi: 10.2135/cropsci2014.09.0627
[27] ISF. Guidelines for Handling Disputes on Essential Derivation of Maize Lines. 2014 ( Adopted by the ISF maize and sorghum section in Beijing, in May 2014), (www.worldseed.org).
[28] Nelson B K, Kahler A L, Kahler J L, Mikel M A, Thompson S A, Ferriss R S, Smith S, Jones E S. Evaluation of the numbers of single nucleotide polymorphisms required to measure genetic distance in maize (Zea may L.). Crop Sci, 2011,51:1470-1480.
doi: 10.2135/cropsci2010.07.0401
[29] Kahler A L, Kahler J L, Thompson S A, Ferriss R S, Jones E S, Nelson B K, Mikel M A, Smith S. North American study on essential derivation in maize: II. Selection and evaluation of a panel of simple sequence repeat loci. Crop Sci, 2010,50:486-503.
[30] ISF. Guidelines for handling disputes on essential derivation of maize lines. 2008 ( Adopted by the ISF maize and sorghum section in Prague, May 2008), (www.worldseed.org).
[31] 王凤格, 赵久然, 郭景伦, 佘花娣, 陈刚. 比较三种DNA指纹分析方法在玉米品种纯度及真伪鉴定中的应用. 分子植物育种, 2003,1:655-661.
Wang F G, Zhao J R, Guo J L, She H D, Chen G. Comparison of three DNA fingerprint analyzing methods for maize cultivars’ identification. Mol Plant Breed, 2003,1:655-661 (in Chinese with English abstract).
[1] 肖颖妮, 于永涛, 谢利华, 祁喜涛, 李春艳, 文天祥, 李高科, 胡建广. 基于SNP标记揭示中国鲜食玉米品种的遗传多样性[J]. 作物学报, 2022, 48(6): 1301-1311.
[2] 崔连花, 詹为民, 杨陆浩, 王少瓷, 马文奇, 姜良良, 张艳培, 杨建平, 杨青华. 2个玉米ZmCOP1基因的克隆及其转录丰度对不同光质处理的响应[J]. 作物学报, 2022, 48(6): 1312-1324.
[3] 胡文静, 李东升, 裔新, 张春梅, 张勇. 小麦穗部性状和株高的QTL定位及育种标记开发和验证[J]. 作物学报, 2022, 48(6): 1346-1356.
[4] 王丹, 周宝元, 马玮, 葛均筑, 丁在松, 李从锋, 赵明. 长江中游双季玉米种植模式周年气候资源分配与利用特征[J]. 作物学报, 2022, 48(6): 1437-1450.
[5] 杨欢, 周颖, 陈平, 杜青, 郑本川, 蒲甜, 温晶, 杨文钰, 雍太文. 玉米-豆科作物带状间套作对养分吸收利用及产量优势的影响[J]. 作物学报, 2022, 48(6): 1476-1487.
[6] 陈静, 任佰朝, 赵斌, 刘鹏, 张吉旺. 叶面喷施甜菜碱对不同播期夏玉米产量形成及抗氧化能力的调控[J]. 作物学报, 2022, 48(6): 1502-1515.
[7] 徐田军, 张勇, 赵久然, 王荣焕, 吕天放, 刘月娥, 蔡万涛, 刘宏伟, 陈传永, 王元东. 宜机收籽粒玉米品种冠层结构、光合及灌浆脱水特性[J]. 作物学报, 2022, 48(6): 1526-1536.
[8] 单露英, 李俊, 李亮, 张丽, 王颢潜, 高佳琪, 吴刚, 武玉花, 张秀杰. 转基因玉米NK603基体标准物质研制[J]. 作物学报, 2022, 48(5): 1059-1070.
[9] 于春淼, 张勇, 王好让, 杨兴勇, 董全中, 薛红, 张明明, 李微微, 王磊, 胡凯凤, 谷勇哲, 邱丽娟. 栽培大豆×半野生大豆高密度遗传图谱构建及株高QTL定位[J]. 作物学报, 2022, 48(5): 1091-1102.
[10] 许静, 高景阳, 李程成, 宋云霞, 董朝沛, 王昭, 李云梦, 栾一凡, 陈甲法, 周子键, 吴建宇. 过表达ZmCIPKHT基因增强植物耐热性[J]. 作物学报, 2022, 48(4): 851-859.
[11] 刘磊, 詹为民, 丁武思, 刘通, 崔连花, 姜良良, 张艳培, 杨建平. 玉米矮化突变体gad39的遗传分析与分子鉴定[J]. 作物学报, 2022, 48(4): 886-895.
[12] 闫宇婷, 宋秋来, 闫超, 刘爽, 张宇辉, 田静芬, 邓钰璇, 马春梅. 连作秸秆还田下玉米氮素积累与氮肥替代效应研究[J]. 作物学报, 2022, 48(4): 962-974.
[13] 刘丹, 周彩娥, 王晓婷, 吴启蒙, 张旭, 王琪琳, 曾庆东, 康振生, 韩德俊, 吴建辉. 利用集群分离分析结合高密度芯片快速定位小麦成株期抗条锈病基因YrC271[J]. 作物学报, 2022, 48(3): 553-564.
[14] 徐宁坤, 李冰, 陈晓艳, 魏亚康, 刘子龙, 薛永康, 陈洪宇, 王桂凤. 一个新的玉米Bt2基因突变体的遗传分析和分子鉴定[J]. 作物学报, 2022, 48(3): 572-579.
[15] 宋仕勤, 杨清龙, 王丹, 吕艳杰, 徐文华, 魏雯雯, 刘小丹, 姚凡云, 曹玉军, 王永军, 王立春. 东北主推玉米品种种子形态及贮藏物质与萌发期耐冷性的关系[J]. 作物学报, 2022, 48(3): 726-738.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
京ICP备15008789号-2