Table 1

Primer used in this study"

Fig. 1

Agarose gel electrophoresis of cDNA full length of HcWRKY71 M: DNA marker 2K Plus II; 1: PCR product."

Fig. 2

Nucleotide sequence and encoded amino acids sequence of HcWRKY71 gene The sequence of WRKYGQK motif is highlighted in red box, that of C2H2 motif (CX4CX23HXH) in black box."

Fig. S1

Conserved domain prediction of HcWRKY71 protein in kenaf"

Fig. S2

Prediction of phosphorylation sites of transcription factor HcWRKY71"

Fig. 3

Amino acid sequence alignment of HcWRKY71 and WRKY71 proteins from other plants The different color indicates that the different levels of the conservation amino acid residues. The blue means amino acids are complete conservation, the pink, cyan, yellow means that the conservation of amino acids is no less than 75%, no less than 50%, no less than 33% and white means no more than 33%."

Fig. 4

Phylogenetic tree of HcWRKY71 and WRKY71 from other plants"

Fig. 5

Expression of HcWRKY71 under salt, drought and cadmium stress A: salt stress; B: drought stress; C: cadmium stress. *, ** mean significant difference at the 0.05 and 0.01 probability levels, respectively. Error bars represent the standard error of each treating group (n = 3)."

Fig. 6

HcWRKY71 expression in different organs under salt, drought and cadmium stress A: salt stress; B: drought stress; C: cadmium stress. *, ** mean significant difference at the 0.05 and 0.01 probability levels, respectively. Error bars represent the standard error of each treating group (n = 3)."

Fig. 7

Identification of transgenic Arabidopsis A: identification of transgenic Arabidopsis by hygromycin; B: PCR identification of transgenic Arabidopsis plant; C: real time quantitative PCR identification. *, ** mean significant difference at the 0.05 and 0.01 probability levels, respectively. Error bars represent the standard error of each treating group (n = 3)."