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Acta Agron Sin ›› 2017, Vol. 43 ›› Issue (11): 1611-1621.doi: 10.3724/SP.J.1006.2017.01603

• CROP GENETICS & BREEDING·GERMPLASM RESOURCES·MOLECULAR GENETICS • Previous Articles     Next Articles

Mapping of QTL for Heading Date and Plant Height Using MAGIC Populations of Rice

SHEN Cong-Cong1,2,ZHU Ya-Jun2,CHEN Kai2,CHEN Hui-Zhen3,WU Zhi-Chao1,2,MENG Li-Jun2,XU Jian-Long1,2,4,*   

  1. 1Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; 2Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Shenzhen 518210, China; 3 Pingxiang Institute of Agricultural Science, Pingxiang 337000, China; 4Shenzhen Institute of Breeding & Innovation, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China?
  • Received:2017-03-29 Revised:2017-07-23 Online:2017-11-12 Published:2017-08-01
  • Contact: 徐建龙, E-mail: xujlcaas@126.com E-mail:shencc0805@163.com
  • Supported by:

    This study was supported by the National High Technology Research and Development Program of China (863 Program) (2014AA10A601), The Program of Introducing International Super Agricultural Science and Technology(948 Program) (2016-X16), Shenzhen Peacock Plan (20130415095710361), and the Scientific and Technological Innovation Project of Chinese Academy of Agricultural Sciences.

Abstract:

Association analysis was performed to map QTL for heading date (HD) and plant height (PH) using three multi-parent advanced generation inter-cross (MAGIC) populations including two four-parent- and one eight-parent- derived populations (DC1, DC2 and 8way), and their composite populations (DC12=DC1+DC2, RMPRIL=DC12+8way) derived from eight elite indica lines. Using the phenotypic data of HD collected in the two sites (Jiangxi and Shenzhen) across two years (2015 and 2016), PH collected in the two sites (Jiangxi and Shenzhen) in 2016, and SNP genotypic data of Rice 55K SNP chip, three HD QTLs, qHD3, qHD6 and qHD8 were identified in the same regions of DTH3, Hd3a and Ghd8, respectively. And five PH QTLs (qPH1.1, qPH1.2, qPH1.3, qPH4 and qPH6) were also identified, of which qPH1.1 and qPH1.2 were located in the adjacent regions of Psd1 and sd1. The other three PH QTLs were new loci which were detected only in one population under one environment showing larger effects of genetic background and environment. Different MAGIC populations had different mapping effects for HD and PH. Using eight-parent-derived populations such as 8way, DC12 and RMPRIL not only detected more HD and PH QTL (5, 5, and 6) but also had higher mapping resolution than four parents-derived populations such as DC1 and DC2 (2 and 4), showing an obvious advantage in two aspects of QTL mapping: highly significant level and closed to known genes. Especially, joint analyses of different populations such as DC12 and RMPRIL had significant advantage in QTL mapping. The results laid the foundation for mining the favorable allele of heading date and plant height, and provided materials and information of molecular breeding.

Key words: Rice, MAGIC population, Heading date, Plant height, Association analysis, Quantitative trait locus/loci (QTL)

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