水稻类受体激酶基因<italic>OsBAK1L</italic> 调控细胞坏死机制

水稻类受体激酶基因OsBAK1L 调控细胞坏死机制

陈现朝, 黄立钰, 周永力^*

Mechanism of Receptor-like Kinases GeneOsBAK1L Involved in Regulating Cell Death Response of Rice

CHEN Xian-Chao, HUANG Li-Yu, ZHOU Yong-Li^*

图6 OsBAK1 -RNAi转基因苗PCR鉴定和 OsBAK1L 基因表达分析 A: 上部是载体35S启动子引物和目的基因引物扩增 OsBAK1L -RNAi片段; 下部是扩增选择标记 Bar 基因。 T: OsBAK1L -RNAi转基因阳性苗; C: 9804- Rxo1 ; V+: pB7GWIWG2II- OsBAK1L 质粒; V: pB7GWIWG2II。B: OsBAK1L 表达分析; 1~5: 分别是接细菌性条斑病菌Rs105后24 h、48 h、72 h、96 h、120 h的T 0 转基因植株; C: 9804- Rxo1 。
Fig. 6 Identification of the OsBAK1 -RNAi transgene rice plants by PCR and the expression of OsBAK1L A: upside shows amplification of the segment of OsBAK1L -RNAi by 35S and OsBAK1L as forward and reward primers; down side shows PCR of Bar gene.T: transgene rice of OsBAK1L -RNAi; C: 9804- Rxo1 ; V+: pB7GWIWG2II- OsBAK1L ; V: pB7GWIWG2II. B: semi-quantitative PCR analysis of OsBAK1L ; 1-5: T 0 transgenic rice of OsBAK1L -RNAi at 24 h, 48 h, 72 h, 96 h, 120 h after inoculating X. o . pv. oryzicola Rs105, respectively; C: 9804- Rxo1 .