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酵母表面展示系统的改进及其在筛选烟草PMT基因启动子结合蛋白中的应用
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陈红, 牛海峡, 王文静, 马浩然, 李加纳, 柴友荣, 张洪博#cod#x0002A; |
Screening of Promoter-Binding Factors of TobaccoPMT Gene Using a Modified Yeast Surface Display System
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CHEN Hong, NIU Hai-Xia, WANG Wen-Jing, MA Hao-Ran, LI Jia-Na, CHAI You-Rong, ZHANG Hong-Bo #cod#x0002A;
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图7 ERF转录因子与GAG片段在酵母中的互作分析 A: 酵母在SD-Leu-Ura-His培养基上的生长; B: 酵母在SD-Leu-Ura-His3-AT培养基上的生长; C: #cod#x003b2;-半乳糖苷酶活性分析; D: 平板上的酵母菌株分布图; MYC2a为GAG结合蛋白对照。 Fig. 7 Interaction in vivo between ERF transcription factors and GAG fragment A: growth of yeast on SD-Leu-Ura-His plate; B: growth of yeast on SD-Leu-Ura-His3-AT plate; C: #cod#x003b2;-galactopyranoside activity assay; D: labeling of yeast strains; MYC2a is used as a control that binds GAG fragment. |
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