小麦籽粒淀粉分支酶同工酶结构组成及时空表达
刘正帅1, 刘贵芬1, 杨明煜2, 贾晓1, 李运祥1, 赵法茂1,#cod#x0002A;

Constitution and Spatiotemporal Expression of Starch Branching Enzyme in Developing Wheat Grain
LIU Zheng-Shuai1, LIU Gui-Fen1, YANG Ming-Yu2, JIA Xiao1, LI Yun-Xiang1, ZHAO Fa-Mao1,#cod#x0002A;
图2 小麦不同灌浆阶段籽粒SBE同工酶的Native-PAGE图谱 a: 开花后5 d; b: 开花后20 d; c: 开花后30 d。花后5 d, 同工酶A未表达酶活性染色为蓝色, 第一条带为内源磷酸化酶; 花后20 d和30 d, 同工酶A已经表达酶活性染色为褐色, 但与内源磷酸化酶带重叠。1: 鄂麦6号; 2: 陕旱8675; 3: 济南16; 4: 石特14; 5: 鲁麦18; 6: 山东辐63; 7: 济南17; 8: 周麦19。
Fig. 2 Native-PAGE profile of SBE isozymes in wheat grain during filling period a: at 5 d after anthesis DAA; b: at 20 DAA; c: at 30 DAA. At 5 DAA, the most upper band shows phosphorylase because isozyme A was not expressed The color of band was blue. At 20 DAA and 30 DAA, the most upper band shows the superposed phosphorylase and SBE isozyme A Enzyme activity staining showed brown. 1: Emai 6; 2: Shaanhan 8675; 3: Jinan 16; 4: Shite 14; 5: Lumai 18; 6: Shandongfu 63; 7: Jinan 17; 8: Zhoumai 19.