比较和分析<italic>NtGNL1</italic>的反义寡聚核苷酸抑制在烟草三种培养体系中的效果

比较和分析NtGNL1的反义寡聚核苷酸抑制在烟草三种培养体系中的效果

廖芳蕾¹, 王鲁², 辛可行¹, 陈文荣¹, 郭卫东^1,^*

Comparison and Analysis of Effects of Antisense Oligodeoxynucleotide Inhibition ofNtGNL1 in Three Culture Systems of Tobacco

LIAO Fang-Lei¹, WANG Lu², XIN Ke-Xing¹, CHEN Wen-Rong¹, GUO Wei-Dong^1,^*

图1 ON4在胚珠离体培养中的应用 A: 对照; B: ON4处理的胚珠。ON4处理4 d之后取图。C, D, E, F: 反义ODN处理的胚珠中的胚胎形态。D: 胚胎发育不对称; E: 胚胎细胞隆起; F: 正常形态。Bar=20 #cod#x003bc;m
Fig. 1 Application of ON4 to the in vitro cultured ovules A: control; B: ON4 treatment. Pictures were taken after four days in the ON4 treatment. Bar=200 #cod#x003bc;m。C, D, E, and F are the embryos morphology in the ovules in the ON4 treatment. D: asymmetrical embryo; E: part embryonic cells bulged from the embryo; F: normal phenotype under the ON4 treatment. Bar=20 #cod#x003bc;m.