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比较和分析NtGNL1的反义寡聚核苷酸抑制在烟草三种培养体系中的效果
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廖芳蕾1, 王鲁2, 辛可行1, 陈文荣1, 郭卫东1,* |
Comparison and Analysis of Effects of Antisense Oligodeoxynucleotide Inhibition ofNtGNL1 in Three Culture Systems of Tobacco
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LIAO Fang-Lei 1, WANG Lu 2, XIN Ke-Xing 1, CHEN Wen-Rong 1, GUO Wei-Dong 1,*
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图3 反义ODN 5 #cod#x003bc;mol L -1 在短期内抑制 NtGNL1 基因的mRNA表达 种子与反义ODN共培养7 d后, 目标基因的表达在ON4和ON6的处理下有不同程度的下调; 种子萌发后转到土壤中7 d, 目标基因的表达并无影响。 Fig. 3 Effect of antisense ODN treatment on NtGNL1 mRNA expression The mRNA expression of NtGNL1 was down regulated after the seeds were co-cultured with antisense ODN for seven days, while the mRNA expression was not influenced when those germinated seeds were transplanted to the soil for seven days. |
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