两个水稻品种镉积累相关基因表达及其分子调控机制

黄志熊^1,², 王飞娟², 蒋晗², 李志兰³, 丁艳菲², 江琼², 陶月良⁴, 朱诚^1,^2,^*

Comparison of Cadmium-Accumulation-Associated Genes Expression and Molecular Regulation Mechanism between Two Rice Cultivars (Oryza sativa L. subspeciesjaponica)

HUANG Zhi-Xiong^1,², WANG Fei-Juan², JIANG Han², LI Zhi-Lan³, DING Yan-Fei², JIANG Qiong², TAO Yue-Liang⁴, ZHU Cheng^1,^2,^*

图1 不同生长发育期的秀水110和秀水11叶片中 OsPCR1 基因的表达水平提取A营养生长期5 #cod#x003bc;mol L -1 Cd处理1 d、B抽穗前期5 #cod#x003bc;mol L -1 Cd处理35 d、C抽穗期5 #cod#x003bc;mol L -1 Cd处理60 d、D成熟期5 #cod#x003bc;mol L -1 Cd处理105 d和E完全成熟期5 #cod#x003bc;mol L -1 Cd处理130 d水稻品种倒数第2叶和第3叶总RNA, 没有Cd处理的样本为对照组。利用qRT-PCR检测 OsPCR1 基因表达水平。数据以 OsUBQ5 为内参进行标准化定量, 以各自秀水110的样本为参照, 以平均值#cod#x000b1;标准误表示mean #cod#x000b1; SE n = 3~8。星号表示秀水110和秀水11之间具有显著差异 * P 0.05; ** P 0.01; *** P 0.001; t 检验。 Cd+: 5 #cod#x003bc;mol L -1 CdCl 2 处理; Cd-: 对照。
Fig. 1 OsPCR1 expression in leaves of Xiushui 110 and Xiushui 11 at five developmental stages of rice RNA was isolated from the penultimate and antepenultimate leaves of the two cultivars at different development stages, including A vegetative stage, B pre-heading stage, C heading stage, D maturity stage, and E full-ripe stage. Cultivars at these stages were treated with 5 #cod#x003bc;mol L -1 CdCl 2 for 1 d, 35 d, 60 d, 105 d, and 130 d, respectively, and cultivars without CdCl 2 treatment were used as control. OsPCR1 expression was determined by qRT-PCR. Relative OsPCR1 mRNA expression was normalized with OsUBQ5 as reference and relative to the Xiushui 110 samples, respectively. The data are presented as mean #cod#x000b1; SE n = 3 to n = 8. Asterisks represent a significant difference between Xiushui 11 and Xiushui 110 * P 0.05; ** P 0.01; *** P 0.001; t -test. Cd+: with 5 #cod#x003bc;mol L -1 CdCl 2 treatment; Cd-: control.