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两个水稻品种镉积累相关基因表达及其分子调控机制
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黄志熊1,2, 王飞娟2, 蒋晗2, 李志兰3, 丁艳菲2, 江琼2, 陶月良4, 朱诚1,2,* |
Comparison of Cadmium-Accumulation-Associated Genes Expression and Molecular Regulation Mechanism between Two Rice Cultivars (Oryza sativa L. subspeciesjaponica)
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HUANG Zhi-Xiong 1,2, WANG Fei-Juan 2, JIANG Han 2, LI Zhi-Lan 3, DING Yan-Fei 2, JIANG Qiong 2, TAO Yue-Liang 4, ZHU Cheng 1,2,*
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图2 不同生长发育期的秀水110和秀水11叶片中siRNA基因的表达水平 利用qRT-PCR技术检测水稻品种倒数第2叶和第3叶总siRNA表达水平。数据以U6核小RNA为内参进行标准化定量, 以各自秀水110的样本为参照定义为1, 以平均值#cod#x000b1;标准误表示mean #cod#x000b1; SE n = 3~4。A~E对应时期, 标注及缩写同图1。 Fig. 2 siRNA expression in leaves of Xiushui 110 and Xiushui 11 at five developmental stages of rice qRT-PCR was performed to detect the expression of the siRNA in the penultimate and antepenultimate leaves of the two cultivars. Relative expression of siRNA in the leaves of Xiushui 11 was normalized to that in the leaves of Xiushui 110, which is defined as 1. U6 small nuclear RNA was used for normalization. The data are presented as mean #cod#x000b1; SE . n = 3 and n = 4. The stages in individual figure, symbols and abbreviation are the same as those given in Fig. 1. |
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