结球甘蓝雌蕊发育调控因子SPT与HEC1的基因克隆及相互作用

许俊强, 孙梓健, 刘智宇, 杨朴丽, 汤青林, 王志敏, 宋明^*, 王小佳^*

Gene Cloning and Interaction between Transcription Factors SPT and HEC1 for Pistil Development inBrassica oleracea L. var. capitataL.

XU Jun-Qiang, SUN Zi-Jian, LIU Zhi-Yu, YANG Pu-Li, TANG Qing-Lin, WANG Zhi-Min, SONG Ming^*, WANG Xiao-Jia^*

图5 pCold I-SPT和pGEX-HEC1原核表达产物的体外相互 br 作用检测 M: 蛋白分子量标准; 1: 未诱导pGEX-HEC1; 2: 未诱导pCold I-SPT; 3: 诱导pGEX-HEC1融合蛋白; 4: 诱导pCold I-SPT融合蛋白; 5: 纯化的pCold I-SPT融合蛋白; 6: 纯化的pGEX-HEC1融合蛋白; 7~8: pET43.1a-SPT融合蛋白与pET43.1a-HEC1eSRK7融合蛋白孵育结果。
Fig. 5 Interaction between pCold I-SPT and pGEX-HEC1 br in vitro M: protein standard; 1: pGEX-HEC1 induced without IPTG; br 2: pCold I-SPT induced without IPTG; 3: pGEX-HEC1 induced with IPTG; 4: pCold I-SPT induced without IPTG; 5: purified 6His-tagged pCold I-SPT; 6: purified GST-tagged pCold I-SPT; br 7-8: interaction between pGEX-HEC1 and pCold I-SPT.