结球甘蓝雌蕊发育调控因子SPT与HEC1的基因克隆及相互作用

许俊强, 孙梓健, 刘智宇, 杨朴丽, 汤青林, 王志敏, 宋明^*, 王小佳^*

Gene Cloning and Interaction between Transcription Factors SPT and HEC1 for Pistil Development inBrassica oleracea L. var. capitataL.

XU Jun-Qiang, SUN Zi-Jian, LIU Zhi-Yu, YANG Pu-Li, TANG Qing-Lin, WANG Zhi-Min, SONG Ming^*, WANG Xiao-Jia^*

图8 SPT和HEC1酵母相互作用检测
Fig. 8 Analysis of interactions between SPT and HEC1in yeast on the SD-Ade-His-Leu-TrpX-#cod#x003b1;-galAbA plate 5: Y2HGold pGBKT7 #cod#x000d7; Y187pGADT7-SPT; 6: Y2HGold pGBKT7 #cod#x000d7; Y187pGADT7-HEC1; 7: Y187 pGADT7 #cod#x000d7; Y2HGold pGBKT7-SPT; 8: Y187pGADT7 #cod#x000d7; Y2Hgold pGBKT7-HEC1; 9: Y2HGold pGBKT7-SPT #cod#x000d7; Y187 pGADT7-HEC1; 10: Y2HGold pGBKT7-HEC1 #cod#x000d7; Y187 pGADT7-SPT; 11: Y2HGold pGBKT7-Lam #cod#x000d7; Y187pGADT-T; 12: Y2HGold pGBKT7-T53 #cod#x000d7; Y187 pGADT-T.