一年生簇毛麦α-醇溶蛋白基因的分离、原核表达与功能鉴定

杨帆¹, 陈其皎^1,², 高翔^1,^2,^*, 赵万春^1,^2,^*, 强琴琴¹, 吴丹¹, 孟敏¹

Cloning, Prokaryotic Expression andin vitro Functional Analysis of α-Gliadin Genes fromDasypyrum villosum

YANG Fan¹, CHEN Qi-Jiao^1,², GAO Xiang^1,^2,^*, ZHAO Wan-Chun^1,^2,^*, JIANG Qin-Qin¹, WU Dan¹, MENG Min¹

图4 蛋白表达和纯化的SDS-PAGE分析 A: 诱导表达产物的SDS-PAGE分析; B: 纯化产物的SDS-PAGE分析。M: Blue Plus protein marker; 1: 未诱导的表达菌株DE3; 2: 未诱导空载体; 3: 诱导空载体; 4和6: 未诱导的重组质粒; 5和7: 诱导后的重组质粒。箭头示目的蛋白。
Fig. 4 SDS-PAGE analysis of protein expression and protein purification A: analysis of expressed proteins by SDS-PAGE; B: analysis of purified proteins by SDS-PAGE. M: Blue Plus protein marker; 1: protein of BL21 DE3 expression strain without induction; 2, protein of non-recombinant plasmid without induction; 3, protein of non-recombinant plasmid after induction; 4 and 6, protein of recombinant plasmid without induction; 5 and 7, protein of recombinant plasmid after induction. Target proteins are indicated by arrows.