小麦自噬相关基因<italic>ATG10</italic>的克隆及白粉菌侵染诱导的<italic>ATG10</italic>的表达

小麦自噬相关基因ATG10的克隆及白粉菌侵染诱导的ATG10的表达

张微¹, 孙鸿¹, 邢莉萍², 卫晓静¹, 王华忠^1,^*

Cloning of Autophagy-related Genes,ATG10s, in Wheat and Their Expression Characteristics Induced byBlumeria graminis f. sp.tritici

ZHANG Wei¹, SUN Hong¹, XING Li-Ping², WEI Xiao-Jing¹, WANG Hua-Zhong^1,^*

图8 TaATG10b的原核表达和纯化 M: 分子量标准; 1: 未诱导对照; 2: IPTG诱导2 h; 3: IPTG诱导6.5 h; 4~8: TaATG10b的Ni 柱亲和层析过程收集液, 其中4为流穿液; 5和6为300 mmol L -1 咪唑洗脱第1、第2管收集液; 7和8为500 mmol L -1 咪唑洗脱第1、第2管收集液。箭头示目标蛋白。
Fig. 8 Prokaryotic expression and purification of TaATG10b M: protein ruler; 1: E. coli lysate of non-induction control; 2: induced by IPTG for 2 h; 3: induced by IPTG for 6.5 h; 4-8: collections in the process of TaATG10b purification by Ni affinity chromatography, including flow-through fraction 4, the first 5 and second 6 one-milliliter elute of 300 mmol L -1 imidazole and the first 7 and second 8 one-milliliter elute of 500 mmol L -1 imidazole. The arrow indicates the expressed product of TaATG10b .