|
小麦自噬相关基因ATG10的克隆及白粉菌侵染诱导的ATG10的表达
|
张微1, 孙鸿1, 邢莉萍2, 卫晓静1, 王华忠1,* |
Cloning of Autophagy-related Genes,ATG10s, in Wheat and Their Expression Characteristics Induced byBlumeria graminis f. sp.tritici
|
ZHANG Wei 1, SUN Hong 1, XING Li-Ping 2, WEI Xiao-Jing 1, WANG Hua-Zhong 1,*
|
|
图8 TaATG10b的原核表达和纯化 M: 分子量标准; 1: 未诱导对照; 2: IPTG诱导2 h; 3: IPTG诱导6.5 h; 4~8: TaATG10b的Ni 柱亲和层析过程收集液, 其中4为流穿液; 5和6为300 mmol L -1 咪唑洗脱第1、第2管收集液; 7和8为500 mmol L -1 咪唑洗脱第1、第2管收集液。箭头示目标蛋白。 Fig. 8 Prokaryotic expression and purification of TaATG10b M: protein ruler; 1: E. coli lysate of non-induction control; 2: induced by IPTG for 2 h; 3: induced by IPTG for 6.5 h; 4-8: collections in the process of TaATG10b purification by Ni affinity chromatography, including flow-through fraction 4, the first 5 and second 6 one-milliliter elute of 300 mmol L -1 imidazole and the first 7 and second 8 one-milliliter elute of 500 mmol L -1 imidazole. The arrow indicates the expressed product of TaATG10b . |
|
|
![](img_8.png) |
|
|