飞机草类黄酮3’-羟化酶基因(<em> CoF3</em>’<em> H</em>)的克隆及其在烟草中的表达

飞机草类黄酮3’-羟化酶基因( CoF3’ H)的克隆及其在烟草中的表达

何海旺, 潘华清, 张铙丹, 何龙飞^*

Chromolaena odorata Flavonoid 3’-hydroxylase Gene Cloning and Its Expression in Tobacco

HE Hai-Wang, PAN Hua-Qing, ZHANG Nao-Dan, HE Long-Fei^*

图1 飞机草 CoF3’H 基因的克隆 M: marker; 1: CoF3’H 中间片段的第2轮扩增产物; 2: CoF3’H 的3°未端的第2轮扩增产物; 3: CoF3’H 的5°未端的扩增产物; D: CoF3’H ORF全长的扩增产物。
Fig. 1 Cloning of CoF3’H gene from C. odorata M: marker; 1: The second round PCR products of middle fragment; 2: The second round PCR products of 3°-end; 3: Amplification of 5°-end; 4: Amplification of ORF.