以谷蛋白GluA-2信号肽增强外源蛋白在转基因水稻胚乳中的表达与积累

王红梅¹, 张昌泉¹, 李钱峰¹, 辛世文², 刘巧泉^1,^*, 徐明良^1,³

Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of Glutelin GluA-2 in Endosperm of Transgenic Rice

WANG Hong-Mei¹, ZHANG Chang-Quan¹, LI Qian-Feng¹, SUN Samuel Sing-Min², LIU Qiao-Quan^1,^*, XU Ming-Liang^1,³

图2 转基因水稻的PCR a和未成熟种子总RNA的Northern杂交分析b 图a为转基因水稻植株总DNA的PCR分析, 所用引物为 GUS 基因特异引物。图b上图为Northern杂交结果, 下图显示总RNA中的28S核糖体RNA。总RNA分别抽提自GG和GSG类转基因水稻植株或未转化植株标记为WT的未成熟种子。杂交所用的探针为地高辛标记的反义 GUS 编码区片段。
Fig. 2 PCR a and Northern blot b analyses of transgenic rice Panels a and b show the PCR analysis using GUS specific primers of total leaf DNA and Northern blot analysis of total RNA from developing seeds of rice plants, respectively. WT means wild type plant, GG and GSG mean the transgenic plants derived from the GG and GSG constructs, respectively. The probe for Northern blot is DIG-labeled anti- GUS DNA fragment.