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以谷蛋白GluA-2信号肽增强外源蛋白在转基因水稻胚乳中的表达与积累
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王红梅1, 张昌泉1, 李钱峰1, 辛世文2, 刘巧泉1,*, 徐明良1,3 |
Enhancing Expression and Accumulation of Foreign Proteins by Using the Signal Peptide of Glutelin GluA-2 in Endosperm of Transgenic Rice
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WANG Hong-Mei 1, ZHANG Chang-Quan 1, LI Qian-Feng 1, SUN Samuel Sing-Min 2, LIU Qiao-Quan 1,*, XU Ming-Liang 1,3
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图4 转基因水稻植株成熟种子蛋白的Western杂交分析 种子蛋白分别抽提自GG和GSG类转基因水稻植株或未转化植株WT的成熟种子, 其中a为种子总蛋白, b为种子可溶性蛋白, c为去除可溶性蛋白后的其他蛋白。每个泳道所加的蛋白量一致。图左侧箭头所指为GUS蛋白。 Fig. 4 Western blot analyses of proteins from mature seeds of transgenic rice Proteins were extracted from the mature seeds of GG and GSG transgenic plants or their wild type WT, respectively. Panels a, b, and c mean the total proteins, soluble proteins and the remaining total proteins after extraction of soluble proteins, respectively. The amount of proteins for each lane is same. The arrow at left indicates the expressed GUS protein. |
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