甘蔗转录激活因子<em> ScCBF1</em>基因的克隆与表达分析

甘蔗转录激活因子 ScCBF1基因的克隆与表达分析

成伟, 郑艳茹, 葛丹凤, 程光远, 翟玉山, 邓宇晴, 彭磊, 谭向尧, 徐景升^*

Molecular Cloning and Expression Analysis of Transcriptional Activators ScCBF1 Gene from Sugarcane

CHENG Wei, ZHENG Yan-Ru, GE Dan-Feng, CHENG Guang-Yuan, ZHAI Yu-Shan, DENG Yu-Qing, PENG Lei, TAN Xiang-Yao, XU Jing-Sheng^*

pGEX-6P-1-ScCBF1重组质粒双酶切PCR鉴定. M1: 15000+2000 bp 分子量标记; M2: 100 bp marker; 1~2: 重组质粒双酶切片段; 3~4: 质粒PCR目的片段。