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甘蓝BoExo70A1与BoSEC3、BoExo84蛋白相互作用的 酵母双杂交检测
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高启国1,*, 刘豫东1,*, 蒲全明1, 张林成1, 朱利泉2, 王小佳1 |
Interaction Analysis of Brassica oleracea L. BoExo70A1 with BoSEC3 and BoExo84 Proteins by Using Yeast Two-hybird System
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GAO Qi-Guo 1,**, LIU Yu-Dong 1,**, PU Quan-Ming 1, ZHANG Lin-Cheng 1, ZHU Li-Quan 2, WANG Xiao-Jia 1
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酵母重组质粒毒性检测 1: pGBKT7酵母转化子在SD/-Trp平板; 2: pGBKT7-BoExo70A1酵母转化子在SD/-Trp平板; 3: pGADT7-BoSEC3酵母转化子在SD/-Leu平板; 4: pGADT7-BoSEC10酵母转化子在SD/-Leu平板; 5: pGADT7-BoSEC15酵母转化子在SD/-Leu平板上; 6: pGADT7-BoExo84-N酵母转化子在SD/-Leu平板; 7: pGADT7-BoExo84-C酵母转化子在SD/-Leu平板。 |
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