Molecular Cloning and Functional Identification of Peroxidase Gene ScPOD02 in Sugarcane
SU Ya-Chun, WANG Zhu-Qing, LI Zhu, LIU Feng, XU Li-Ping*, QUE You-Xiong, DAI Ming-Jian, Chen Yun-Hao
BL21 pET 32a和BL21 pET 32a-ScPOD02在添加PEG的LB培养基上的平板胁迫验证 BL21 pET 32a和BL21 pET 32a-ScPOD02菌液经IPTG诱导表达后稀释至OD600为0.6, 然后分别稀释1×10-3倍与1×10-4倍。各取10 μL 1×10-3 (LB平板红线左边区域)和1×10-4 (LB平板红线右边区域)菌液点至含0、15%、30%和45% PEG的LB平板培养基上。