以抗除草剂Bar基因稳定转化谷子技术研究
陈倩楠,王轲,汤沙,杜丽璞,智慧,贾冠清,赵宝华,叶兴国,刁现民

Use of Bar Gene for the Stable Transformation of Herbicide-resistant Foxtail Millet Plants
Qian-Nan CHEN,Ke WANG,Sha TANG,Li-Pu DU,Hui ZHI,Guan-Qing JIA,Bao-Hua ZHAO,Xing-Guo YE,Xian-Min DIAO
表1 农杆菌介导谷子愈伤组织转化培养基配方
Table 1 Media composition of Agrobacterium-mediated foxtail millet calli transformation (g L-1)
组分
Composition
诱导培养基
IM
侵染液
IL
共培养基
CM
恢复培养基RM 筛选培养基
SM
分化培养基DM 壮苗培养基
SSM
MS 4.43 0.443 0.443 4.43 4.43 4.43 2.215
L-Glu 0.5 0.5 0.5
CEH 0.8 0.8 0.8 0.8 0.8
MES 1.95 0.5 0.5 1.95 1.95
inositol 0.1 0.1 0.1 0.1
VCa 0.01 0.01
Glucose 36 10
Sucrose 68.5 20 30
Maltose 40 40 40
CuSO4a 0.0006
AgNO3a 0.005 0.005 0.005 0.005
2,4-Da 0.002 0.002 0.002 0.002
KTa 0.0005 0.005 0.0005 0.0005 0.002
NAAa 0.0005
ASa 0.1962, L-1
Cefa 0.1
Carba 0.25 0.25
Tima 0.15 0.15
PPTa 0.005/0.01 0.001
Agar 5 8 5 5
Phytagel 2.5 2