以抗除草剂Bar基因稳定转化谷子技术研究
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陈倩楠,王轲,汤沙,杜丽璞,智慧,贾冠清,赵宝华,叶兴国,刁现民
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Use of Bar Gene for the Stable Transformation of Herbicide-resistant Foxtail Millet Plants
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Qian-Nan CHEN,Ke WANG,Sha TANG,Li-Pu DU,Hui ZHI,Guan-Qing JIA,Bao-Hua ZHAO,Xing-Guo YE,Xian-Min DIAO
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表1 农杆菌介导谷子愈伤组织转化培养基配方
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Table 1 Media composition of Agrobacterium-mediated foxtail millet calli transformation (g L-1)
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组分 Composition | 诱导培养基 IM | 侵染液 IL | 共培养基 CM | 恢复培养基RM | 筛选培养基 SM | 分化培养基DM | 壮苗培养基 SSM | MS | 4.43 | 0.443 | 0.443 | 4.43 | 4.43 | 4.43 | 2.215 | L-Glu | 0.5 | — | — | 0.5 | 0.5 | — | — | CEH | 0.8 | 0.8 | 0.8 | 0.8 | 0.8 | — | — | MES | 1.95 | 0.5 | 0.5 | 1.95 | 1.95 | — | — | inositol | 0.1 | — | — | 0.1 | 0.1 | 0.1 | — | VCa | — | — | — | — | — | 0.01 | 0.01 | Glucose | — | 36 | 10 | — | — | — | — | Sucrose | — | 68.5 | — | — | — | 20 | 30 | Maltose | 40 | — | — | 40 | 40 | — | — | CuSO4a | — | 0.0006 | — | — | — | — | — | AgNO3a | 0.005 | — | 0.005 | 0.005 | 0.005 | — | — | 2,4-Da | 0.002 | — | 0.002 | 0.002 | 0.002 | — | — | KTa | 0.0005 | — | 0.005 | 0.0005 | 0.0005 | 0.002 | — | NAAa | — | — | — | — | — | — | 0.0005 | ASa | — | — | 0.1962, L-1 | — | — | — | — | Cefa | — | — | — | 0.1 | — | — | — | Carba | — | — | — | 0.25 | 0.25 | — | — | Tima | — | — | — | — | — | 0.15 | 0.15 | PPTa | — | — | — | — | 0.005/0.01 | — | 0.001 | Agar | 5 | — | 8 | 5 | 5 | — | — | Phytagel | — | — | — | — | — | 2.5 | 2 |
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