以抗除草剂Bar基因稳定转化谷子技术研究

陈倩楠,王轲,汤沙,杜丽璞,智慧,贾冠清,赵宝华,叶兴国,刁现民

Use of Bar Gene for the Stable Transformation of Herbicide-resistant Foxtail Millet Plants

Qian-Nan CHEN,Ke WANG,Sha TANG,Li-Pu DU,Hui ZHI,Guan-Qing JIA,Bao-Hua ZHAO,Xing-Guo YE,Xian-Min DIAO

图2 愈伤组织诱导培养时间对转化效率的影响 A: 诱导7 d后愈伤组织; B: 诱导15 d后愈伤组织; C: 诱导30 d后愈伤组织; D: GUS瞬时转化效率; E: 诱导15 d愈伤组织GUS染色; F: 诱导30 d愈伤组织GUS染色。*, **分别表示在P ≤ 0.05和P ≤ 0.01水平上的显著差异。

Fig. 2 Effect of calli induction time on transformation efficiency A: calli after induction for 7 d; B: calli after induction for 15 d; C: calli after induction for 30 d; D: GUS transient conversion efficiency; E: GUS staining of calli after induction for 15 d; F: GUS staining of calli after induction for 30 d. * and ** indicates a significant difference at P ≤ 0.05 and P ≤ 0.01 levels, respectively.