以抗除草剂Bar基因稳定转化谷子技术研究

陈倩楠,王轲,汤沙,杜丽璞,智慧,贾冠清,赵宝华,叶兴国,刁现民

Use of Bar Gene for the Stable Transformation of Herbicide-resistant Foxtail Millet Plants

Qian-Nan CHEN,Ke WANG,Sha TANG,Li-Pu DU,Hui ZHI,Guan-Qing JIA,Bao-Hua ZHAO,Xing-Guo YE,Xian-Min DIAO

图3 谷子愈伤组织热激处理GUS瞬时转化 A: 不同温度下热激3 min的GUS瞬时表达率; B: 25°C (CK) GUS染色; C: 45°C热激下GUS染色; D: 45°C热激温度下不同热激时间的GUS瞬时表达率; E: 45°C热激1.5 min GUS染色; F: 45°C热激3 min GUS染色。*和**分别表示在P ≤ 0.05和P ≤ 0.01水平上显著差异。

Fig. 3 Transient expression of GUS after heat shock treatment of foxtail millet calli A: GUS transient expression rate at different temperatures of 3 min; B: GUS staining under CK (25°C); C: GUS staining at 45°C heat shock; D: GUS transient expression rate at different time under 45°C heat shock; E: 45°C heat shock 1.5 min GUS staining; F: 45°C heat shock 3 min GUS staining. * and ** indicates a significant difference at P ≤ 0.05 and P ≤ 0.01 levels, respectively.