以抗除草剂Bar基因稳定转化谷子技术研究

陈倩楠,王轲,汤沙,杜丽璞,智慧,贾冠清,赵宝华,叶兴国,刁现民

Use of Bar Gene for the Stable Transformation of Herbicide-resistant Foxtail Millet Plants

Qian-Nan CHEN,Ke WANG,Sha TANG,Li-Pu DU,Hui ZHI,Guan-Qing JIA,Bao-Hua ZHAO,Xing-Guo YE,Xian-Min DIAO

图4 T0代转基因植株鉴定 A: 愈伤组织分化; B: 再生植株; C: 壮苗; D: 再生植株移栽; E: Bar基因PCR检测, 1~7为T0代转化单株; F: T0代转化株Southern blot, 1~5: 经Sac I酶切, 7~11: 经Hind III酶切, 6: 野生型。M: marker DL2000; H: 水对照; WT: 野生型; P: 质粒。

Fig. 4 Identification of T0 generation transgenic lines A: calli differentiation; B: regenerated plant; C: strong seedling; D: transplanting of regenerated plants; E: bar PCR detection, 1-7 are T0 generation transformed plants; F: Southern blot of T0 generation of transgenic lines, 1-5: digested with Sac I, 7-11: digested with Hind III, 6: wild type. M: marker DL2000; H: water control; WT: wild type; P: plasmid.