以抗除草剂<i>Bar</i>基因稳定转化谷子技术研究

以抗除草剂Bar基因稳定转化谷子技术研究

陈倩楠,王轲,汤沙,杜丽璞,智慧,贾冠清,赵宝华,叶兴国,刁现民

Use of Bar Gene for the Stable Transformation of Herbicide-resistant Foxtail Millet Plants

Qian-Nan CHEN,Ke WANG,Sha TANG,Li-Pu DU,Hui ZHI,Guan-Qing JIA,Bao-Hua ZHAO,Xing-Guo YE,Xian-Min DIAO

图5 T₃代转基因株bar基因抗性试验
A: 2.5 g L^-1PPT溶液涂抹叶片; B: 2.5 g L^-1PPT 溶液喷洒叶片, 左侧转化株, 右侧未转化株; C: Bar蛋白抗体试纸条鉴定结果。CK: 未转化阴性对照; Vegative: 转基因阴性株; Positive: 转基因阳性株。

Fig. 5 Bar gene resistance test of T₃ generation transgenic lines
A: 2.5 g L^-1 PPT solution coated leaves; B: 2.5 g L^-1PPT solution sprayed leaves in transgenic lines (left) and in CK (right); C: Bar protein test results. CK: wild type control; Negative: negative transgenic lines; Positive: positive transgenic line.