普通小麦-大赖草易位系T5AS-7LrL·7LrS分子细胞遗传学鉴定

王林生,张雅莉,南广慧

Molecular and Cytogenetic Identification of Triticum aestivum-Leymus racemosus Translocation Line T5AS-7LrL·7LrS

Lin-Sheng WANG,Ya-Li ZHANG,Guang-Hui NAN

图1 易位系T5AS-7LrL·7LrS荧光原位杂交(2n=44) 有丝分裂中期的顺次GISH-FISH, A图中绿色信号为Fluorescein-l2-dUTP标记的大赖草基因组DNA, B图中的红色信号为TARAM标记的Oligo-pSc119.2-2, 绿色信号为6-FAM标记的Oligo-pAs1-2, 箭头指示易位染色体。

Fig. 1 Fluorescence in situ hybridization (FISH) of translocation line T5AS-7LrL·7LrS (2n = 44) Sequential GISH-FISH of mitotic metaphase chromosomes. In panel A, L. racemosus genomic DNA was labeled with fluorescein-12-dUTP and visualized with green signals. In panel B, Oligo-pSc119.2-2 was labeled with TARAM and visualized with red signals ,and Oligo-pAs1-2 was labeled with 6-FAM and visualized with green signals. The arrows show translocation chromosomes.