不同地理来源MGIII组大豆品种生育期结构分析及E基因型鉴定

江红,孙石,宋雯雯,吴存祥,武婷婷,胡水秀,韩天富

Characterization of Growth Period Structure and Identification of E Genes of MGIII Soybean Varieties from Different Geographic Regions

Hong JIANG,Shi SUN,Wen-Wen SONG,Cun-Xiang WU,Ting-Ting WU,Shui-Xiu HU,Tian-Fu HAN

表1 用于鉴定E1、E2、E3和E4基因型的引物

Table 1 Allele-specific primers for distinguishing recessive alleles from dominant functional alleles at the maturity loci E1, E2, E3, and E4 in soybeans

基因座 Locus 等位基因 Allele 引物序列 Primer sequence (5°-3°) 参考文献 Reference E1 e1-nl/e1-as/e1-fs F: CACTCAAATTAAGCCCTTTCA; R: TCCGATCTCATCACCTTTCC [20], [31] E2 e2 F: ACAGCCTCCCGTGCTG; R: TCCCATGAGTTGCGGAATGG E3 e3-ns F: GTCTTTTGTCCTTGTCATTTGTGT; R: CAAAGCATCATCCAATACCCTCC e3-fs F: GGGATAGTTCTGATGCTGTTCAA; R: CCTTGTATCGATAGCATATGTGCT [31] E3-Mi F: TGGGTCTTCAGTTCAGTTGG; R: TGCTTCCTTTCACTTTCTGATG [31] E3-Ha F: TGGAGGGTATTGGATGATGC; R: CGGTCAAGAGCCAACATGAG e3-tr F: TGGAGGGTATTGGATGATGC; R: GTCCTATACAATTCTTTACGACG E4 e4-oto/e4-tsu/e4-kam/e4-kes F: AGACGTAGTGCTAGGGCTAT; R: GCATCTCGCATCACCAGATCA [23] e4-SORE--1 F: GCATCTCGCATCACCAGATCA; R: GCTCATCCCTTCGAATTCAG [32]