芥菜型油菜毛状根诱导体系构建及TTG1基因功能初步研究
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李隆,程成,伍小方,张大为,刘丽莉,周静,周美亮,张凯旋,严明理
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Construction of Hairy Root Induction System and Functional Analysis of TTG1 Gene in Brassica juncea
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Long LI,Cheng CHENG,Xiao-Fang WU,Da-Wei ZHANG,Li-Li LIU,Jing ZHOU,Mei-Liang ZHOU,Kai-Xuan ZHANG,Ming-Li YAN
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表1 qRT-PCR引物表
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Table 1 List of qRT-PCR primers
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基因
Gene | 名称
Name | 引物
Primer (5°-3°) | PCR长度
PCR length (bp) | | BAN | Anthocyanidin reductase | F: ATCTTCCATGTCGCAACTCC
R: CGAGAGAGATAAACTGCTAG | 200 | | DFR | Dihydroflavonol4-reductase | F: AGGATTCATTGGTTCATGGCTCG
R: TCCGTTTATGGCGTCATCGTAGC | 189 | | ANS | Anthocyanidin synthase | F: GGACAACTTGAGTGGGAAGATTA
R: GCAAACGAAGACACTTAGCGTAC | 132 | | TTG1 | protein (“WD40” or “WDR”) | F: GGCTGTAAGCATATCTGTTCCG
R: GAGAAAGCAATCCCAATCCAATG | 173 | 内参
Internal reference | Actin7 | F: GCTGACCGTATGAGCAAAG
R: AAGATGGATGGACCCGAC | 182 |
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