芥菜型油菜毛状根诱导体系构建及TTG1基因功能初步研究

李隆,程成,伍小方,张大为,刘丽莉,周静,周美亮,张凯旋,严明理

Construction of Hairy Root Induction System and Functional Analysis of TTG1 Gene in Brassica juncea

Long LI,Cheng CHENG,Xiao-Fang WU,Da-Wei ZHANG,Li-Li LIU,Jing ZHOU,Mei-Liang ZHOU,Kai-Xuan ZHANG,Ming-Li YAN

图5 转基因毛状根的鉴定 A: 转GUS基因的3个根系Z1、Z2、Z3的PCR阳性检测。+: pCAMBIA3301质粒; -: 野生型毛状根; B: 转TTG1基因的3个根系T1、 T2、T3的PCR阳性检测。+: pCAMBIA3301-TTG1; -: 野生型毛状根。

Fig. 5 Identification of transgenic hairy roots A: three lines of hairy roots overexpressing GUS gene tested by PCR. +: pCAMBIA3301 plasmid; -: wild type hairy roots; B: three lines of hairy roots overexpressing TTG1 gene tested by PCR. +: pCAMBIA3301-TTG1; -: wild-type hairy roots.