UV-B胁迫下Ca 2+对颠茄生理特性与次生代谢产物的调控研究

卢克欢,刘兴,杨怡,廖志华,吴能表

Effect of Exogenous Ca 2+ on Physiological Characteristics and Secondary Metabolites accumulation of Atropa belladonna L. Seedlings under UV-B Stress

Ke-Huan LU,Xing LIU,Yi YANG,Zhi-Hua LIAO,Neng-Biao WU

表2 荧光定量PCR所需引物

Table 2 Primer pairs for quantitative PCR

基因 Gene 登录号 Accession number 上游引物 Forward primer (5'-3') 下游引物 Reverse primer (5'-3') 退火温度 Annealing temperature (°C) PGK JX154676 TCGCTCTTGGAGAAGGTTGAC CTTGTCCGCAATCACTACATCAG 59.5 PMT AB018570 CCTACTTACCCTACTGGTGTTATC GCGAAAGATGGCAAAATAAAAGC 56.4 H6H JN542537 TTCCACTTGAGCAGAAAGCAAAGC CCTCATGGTCAACTTCCTCACTTCC 56.4 TR I JX155757 TTCTTTGCTTCCCTGCTGCTTC CAGGCCAACCTTAGTATCACACAG 61.4