UV-B胁迫下Ca 2+对颠茄生理特性与次生代谢产物的调控研究
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卢克欢,刘兴,杨怡,廖志华,吴能表
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Effect of Exogenous Ca 2+ on Physiological Characteristics and Secondary Metabolites accumulation of Atropa belladonna L. Seedlings under UV-B Stress
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Ke-Huan LU,Xing LIU,Yi YANG,Zhi-Hua LIAO,Neng-Biao WU
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表4 UV-B胁迫下不同浓度Ca2+对颠茄硝酸还原酶、谷氨酰胺合成酶和谷氨酸脱氢酶活性的影响
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Table 4 Effects of Ca2+ on NR, GS, and GDH activities of Atropa belladonna L. under UV-B stress
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指标
Indicator | 处理组
Group | 处理时间 Treatment time | | 4 d | 8 d | 12 d | 硝酸还原酶 NR
(U g-1 FW h-1) | T0 | 1.290±0.048 c | 1.282±0.117 c | 1.333±0.052 d | | T1 | 1.037±0.022 a | 0.813±0.053 a | 0.557±0.039 a | | T2 | 1.035±0.061 a | 0.939±0.108 a | 0.664±0.040 b | | T3 | 1.121±0.009 b | 1.083±0.030 b | 0.724±0.047 b | | T4 | 1.290±0.018 c | 1.213±0.035 bc | 0.902±0.043 c | | T5 | 1.135±0.013 b | 1.121±0.048 b | 0.753±0.080 b | 谷氨酰胺合成酶 GS
(OD540 mg-1 FW h-1) | T0 | 8.349±0.197 c | 7.885±0.259 d | 7.696±0.670 d | | T1 | 6.555±0.235 a | 4.989±0.767 a | 3.952±0.323 a | | T2 | 6.812±0.297 a | 5.666±0.155 b | 4.323±0.214 ab | | T3 | 7.494±0.285 b | 6.442±0.138 c | 4.883±0.226 bc | | T4 | 8.037±0.231 c | 7.336±0.189 d | 5.454±0.201 c | | T5 | 6.870±0.268 a | 6.104±0.105 bc | 4.592±0.219 ab | 谷氨酸脱氢酶 GDH
(U mg-1 FW min-1) | T0 | 5.659±0.402 a | 5.756±0.658 b | 4.968±0.482 b | | T1 | 6.109±0.589 ab | 4.244±0.579 a | 3.344±0.486 a | | T2 | 6.367±0.841 ab | 5.338±0.486 ab | 4.887±0.402 b | | T3 | 7.138±0.880 b | 5.916±0.990 b | 6.881±0.620 c | | T4 | 6.045±0.780 ab | 8.039±0.678 c | 6.431±0.403 c | | T5 | 5.016±0.696 a | 7.395±0.486 c | 5.338±0.589 b |
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