水稻稻瘟病抗性基因Bsr-d1功能标记的开发和利用

王军,赵婕宇,许扬,范方军,朱金燕,李文奇,王芳权,费云燕,仲维功,杨杰

Development and Application of Functional Markers for Rice Blast Resistance Gene Bsr-d1 in Rice

Jun WANG,Jie-Yu ZHAO,Yang XU,Fang-Jun FAN,Jin-Yan ZHU,Wen-Qi LI,Fang-Quan WANG,Yun-Yan FEI,Wei-Gong ZHONG,Jie YANG

表1 基于变异位点设计的功能标记和测序引物

Table 1 Functional markers and sequencing primers designed based on mutation site

引物名称 Primer name 引物序列 Primer sequence (5'-3') 片段长度 Expected size (bp) CAPs1F AGTCTAGCATCCACCGTTCCAC 313 CAPs1R GTAGGCAGGCAGTGGGATGA CAPs2F TTTTATAGGACAGAGGGAATATGTA 368 CAPs2R GCAGTGGGATGAACCTGTAC Bsr-d1-F AGTCTAGCATCCACCGTTCCAC 1Bsr-d1-R CTTTTCGCTTATACTTATATTTATCAGC 241 1bsr-d1-R CTTTTCGCTTATACTTATATTTATCAGT 241 2Bsr-d1-R CTTTTCGCTTATACTTATATTTATCgGC 241 2bsr-d1-R CTTTTCGCTTATACTTATATTTATCgGT 241 3Bsr-d1-R CTTTTCGCTTATACTTATATTTATCAaC 241 3Bsr-d1-R CTTTTCGCTTATACTTATATTTATCAaT 241 C1F AGTCTAGCATCCACCGTTCCAC 755 C1R ATGATTTGATGGGATTGATTGC C2F TTTTATAGGACAGAGGGAATATGTA 983 C2R GCGAGGTACTCCTCCTTGTTGAT