水稻稻瘟病抗性基因Bsr-d1功能标记的开发和利用

王军,赵婕宇,许扬,范方军,朱金燕,李文奇,王芳权,费云燕,仲维功,杨杰

Development and Application of Functional Markers for Rice Blast Resistance Gene Bsr-d1 in Rice

Jun WANG,Jie-Yu ZHAO,Yang XU,Fang-Jun FAN,Jin-Yan ZHU,Wen-Qi LI,Fang-Quan WANG,Yun-Yan FEI,Wei-Gong ZHONG,Jie YANG

图1 2对引物梯度PCR扩增产物在1.5%的琼脂糖胶电泳结果 M: DL2000; 1~12退火温度分别为: 45.1°C, 45.3°C, 45.9°C, 46.8°C, 47.9°C, 49.1°C, 50.4°C, 51.7°C, 52.8°C, 53.8°C, 54.5°C, 54.8°C。

Fig. 1 Gradient PCR amplification products of Digu using two new designed primers M: DL2000; Annealing temperature of 1-12: 45.1°C, 45.3°C, 45.9°C, 46.8°C, 47.9°C, 49.1°C, 50.4°C, 51.7°C, 52.8°C, 53.8°C, 54.5°C, 54.8°C.