水稻稻瘟病抗性基因Bsr-d1功能标记的开发和利用

王军,赵婕宇,许扬,范方军,朱金燕,李文奇,王芳权,费云燕,仲维功,杨杰

Development and Application of Functional Markers for Rice Blast Resistance Gene Bsr-d1 in Rice

Jun WANG,Jie-Yu ZHAO,Yang XU,Fang-Jun FAN,Jin-Yan ZHU,Wen-Qi LI,Fang-Quan WANG,Yun-Yan FEI,Wei-Gong ZHONG,Jie YANG

图5 Bsr-d1基因等位基因特异标记的梯度PCR扩增结果 M: DL2000; A: 1Bsr-d1引物梯度PCR扩增产物; B: 1bsr-d1引物梯度PCR扩增产物; C: 2Bsr-d1引物梯度PCR扩增产物; D: 2bsr-d1引物梯度PCR扩增产物; E: 3Bsr-d1引物梯度PCR扩增产物; F: 3bsr-d1引物梯度PCR扩增产物; 1~12为地谷的不同引物对在退火温度分别为45.1°C、45.3°C、45.9°C、46.8°C、47.9°C、49.1°C、50.4°C、51.7°C、52.8°C、53.8°C、54.5°C、54.8°C时的扩增产物; 13~24为Nipponbare的不同引物对在退火温度分别为45.1°C、45.3°C、45.9°C、46.8°C、47.9°C、49.1°C、50.4°C、51.7°C、52.8°C、53.8°C、54.5°C、54.8°C时的扩增产物。

Fig. 5 Gradient PCR amplification products of Digu and Nipponbare using allelic-specific PCR markers M: DL2000; A: Gradient PCR amplification products by 1Bsr-d1; B: Gradient PCR amplification products by 1bsr-d1; C: Gradient PCR amplification products by 2Bsr-d1; D: Gradient PCR amplification products by 2bsr-d1; E: Gradient PCR amplification products by 3Bsr-d1; F: Gradient PCR amplification products by 3bsr-d1; 1-12: amplification products of Didu by different primers in annealing temperatures of 45.1°C, 45.3°C, 45.9°C, 46.8°C, 47.9°C, 49.1°C, 50.4°C, 51.7°C, 52.8°C, 53.8°C, 54.5°C, 54.8°C; and 13-24: amplification products of Nipponbare by different primers in annealing temperatures of 45.1°C, 45.3°C, 45.9°C, 46.8°C, 47.9°C, 49.1°C, 50.4°C, 51.7°C, 52.8°C, 53.8°C, 54.5°C, 54.8°C.