水稻矮化大粒突变体sdb1的鉴定与基因定位

陶怡然,熊毓贞,谢佳,田维江,张晓琼,张孝波,周倩,桑贤春,王晓雯

Identification and Gene Mapping of sdb1 Mutant with a Semi-dwarfism and Bigger Seed in Rice

Yi-Ran TAO,Yu-Zhen XIONG,Jia XIE,Wei-Jiang TIAN,Xiao-Qiong ZHANG,Xiao-Bo ZHANG,Qian ZHOU,Xian-Chun SANG,Xiao-Wen WANG

表1 基因定位引物序列

Table 1 Primer sequences used for gene mapping

引物名称 Primer name 上游引物序列 Upstream primer sequence (5'-3') 下游引物序列 Downstream primer sequence (5'-3') J50-3 TGCCTCCTTGTGTGTGGAGT TCCAAGCACTGACTTCATAGC J50-2 CCACCAGTGATCCCTCATACA TCCGAGCGATTCTACTGGTC J50-7 CCTCGTGTGGAATCTCGTTC GTAACAGTAGGTGCGAAGATGG J50-53 GCGTTGTGTGCTCACTGG CCACTTGTCAGGTCTCCATC J50-65 CCGGACGAGCCAATCAG CGGTGGCTTCTCCCAAGG