基于SSR分子标记的Nicotiana tobacum-N. plumbaginifolia异源染色体植株的鉴定与筛选

尚维,赵申清玉,党江波,郭启高,梁国鲁,杨超,张艳,陈益银

Identification and Screening of Nicotiana tobacam-N. plumbaginifolia Heterologous Chromosome Plants Based on SSR Marker

Wei SHANG,Shen-Qing-Yu ZHAO,Jiang-Bo DANG,Qi-Gao GUO,Guo-Lu LIANG,Chao YANG,Yan ZHANG,Yi-Yin CHEN

表1 可在云烟87、N. plumbaginifolia基因组DNA中扩增出差异条带的29对引物

Table 1 Sequences of 29 pairs of SSR primer which can amplify different bonds from Yunyan87 genomic DNA and N. plumbaginifolia genomic DNA

引物编号 Primer No. 正向引物序列 Forward primer (5°-3') 反向引物序列 Reverse primer (5°-3') PT30061 TCGTCCATTTCTTTCTCTCTCA CATAAATAGTTGCTCATTCAATCG PT30372 TGCACATGCTATGACGATTATCT GGTCATTGATCGCCAAGTTT PT30138 AGTTGCAGGATTGTTCGCTT CGACTGCAAGAGTTGGCAAT PT50199 CTCAATCAGCCAAATCCCTC TCTCCATTGTTAGAGTGAAGAGAAA PT20213 TGTGGAGCTCCTTTCTTTGC TCAAATCAACAACAAATCCAAT PT30482 CTTCTCTCTCCACCGCAGAC ACAGTTGGATATGGTGGCGT PY50282 GCCAGCGAGAATGAGTGAGT TTTGTAGCCTCGGCTGATGT PT50178 GATTGCAAAGGCGGTTACAT ACCGGACGGTCCAAACTAAG PT50200 TTCTTCCGGTGATGTTCCTC TCTACACGTGTTCCTGTATCTGTG PT50088 CAACAAATTGGAGTGGAGGG TGCACATCCTCAGCCTACAG PT50022 AAACTTGCTTTCTTTGGACATC TTCATGTTGCAACGAATCCT PT50090 CCTATATGATGAGTTGAATATTGCG TGCTCTATAATCATAACGTTGAAGAAG PT50030 GGGCCAATCCGTGTAGAAAG CCCGATTTAGCACTTGATTGA PT50254 TATAATCCCTCCCTGTGCCA TCGCGGTGAGATACAAAGAA PT30412 CATTTAGCCGGGAACATTCA CATGGGATACACACGCAAAG PT50051 AGATCGATCGGGTGAGTGAG TCACTCCACGCACAGAGAGT PT40035 GAGGTGGAAGTCATCGGAAA CGTCTGTCATACACGCGAAA PT50117 GCAGAATCGCAGATCCAGA GGGAGACAGTGGAGGTAGAGG PT30167 TGATACAGAATATGGCGAACTTT CCGCTTCATCATTGAGGTTT PT50225 TGTGTCCTCGTCAACTGTGG TTTGTTATGCACGCATTGCT PT50243 CCCACTCTCTCTTGCCCATA TCAGAATAGCATGTGGGTGTG PT30272 GAACCTAACCTCGCTCCACA AAATGGTAGCTGCGAGGAGA PT50132 CATTCAGCACGCACCTAAGA ATGCTTCCAAACCTTTCTCG PT30259 CAGCCAAGAGAACCCTTCAG GATTACCCTTCAAATGCCGA PT30241 AAGTCTCGTGTGGTTGCTTT AAAGGGCAATGTGTCTAGCTC PT50081 GTGACCGGAAGACGGTGAT CTTCCGTTGAAACTTCGCAC PT50029 GATAAACTTGGTAGGTCCGGC AGGATGAGCAGGAGCATGAC