基于SSR分子标记的Nicotiana tobacum-N. plumbaginifolia异源染色体植株的鉴定与筛选
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尚维,赵申清玉,党江波,郭启高,梁国鲁,杨超,张艳,陈益银
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Identification and Screening of Nicotiana tobacam-N. plumbaginifolia Heterologous Chromosome Plants Based on SSR Marker
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Wei SHANG,Shen-Qing-Yu ZHAO,Jiang-Bo DANG,Qi-Gao GUO,Guo-Lu LIANG,Chao YANG,Yan ZHANG,Yi-Yin CHEN
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表3 29个N. plumbaginifolia位点在回交后代植株中的分布情况
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Table 3 Distribution of 29 N. plumbaginifolia loci in BC1 plants
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引物/位点
Primer/locus | 扩增植株数量
Plants number | 所占比例
Proportion (%) | 引物/位点
Primer/locus | 扩增植株数量
Plants number | 所占比例
Proportion (%) | | PT30061 | 2 | 1.05 | PT30241 | 19 | 10.00 | | PT20213 | 2 | 1.05 | PT50029 | 24 | 12.63 | | PT30412 | 5 | 2.63 | PT50199 | 24 | 12.63 | | PT50022 | 6 | 3.16 | PT50243 | 28 | 14.74 | | PT50051 | 7 | 3.68 | PT40035 | 34 | 17.89 | | PT50225 | 12 | 6.32 | PT30272 | 34 | 17.89 | | PT30259 | 12 | 6.32 | PT50081 | 34 | 17.89 | | PT30361 | 15 | 7.89 | PT30138 | 34 | 17.89 | | PT50200 | 16 | 8.42 | PT50178 | 34 | 17.89 | | PT50254 | 16 | 8.42 | PT50030 | 38 | 20.00 | | PT30167 | 16 | 8.42 | PT50117 | 38 | 20.00 | | PT30146 | 16 | 8.42 | PT50132 | 38 | 20.00 | | PT50088 | 18 | 9.47 | PY50282 | 39 | 20.53 | | PT30482 | 19 | 10.00 | PT50090 | 58 | 30.53 |
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