基于SSR分子标记的Nicotiana tobacum-N. plumbaginifolia异源染色体植株的鉴定与筛选

尚维,赵申清玉,党江波,郭启高,梁国鲁,杨超,张艳,陈益银

Identification and Screening of Nicotiana tobacam-N. plumbaginifolia Heterologous Chromosome Plants Based on SSR Marker

Wei SHANG,Shen-Qing-Yu ZHAO,Jiang-Bo DANG,Qi-Gao GUO,Guo-Lu LIANG,Chao YANG,Yan ZHANG,Yi-Yin CHEN

图3 部分引物扩增种间远缘杂种基因组DNA图谱 M: marker; ♂: N. plumbaginifolia; ♀: 云烟87八倍体; (1): PT30138; (2): PT50178; (3): PT50090; (4): PT50117; (5): PT50030; (6): PT50132; (7): PT50199; (8): PT50200。细箭头为N. plumbaginifolia的 特异条带; 粗箭头为云烟87八倍体的特异条带。

Fig. 3 Filial generation genomic DNA map amplified by part primers M: marker; ♂: N. plumbaginifolia; ♀: Yunyan 87 octaploid; (1): PT30138; (2): PT50178; (3): PT50090; (4): PT50117; (5): PT50030; (6): PT50132; (7): PT50199; (8): PT50200. Thin arrow: loci of N. plumbaginifolia; thick arrow: loci of Yunyan 87 octaploid.