基于SSR分子标记的Nicotiana tobacum-N. plumbaginifolia异源染色体植株的鉴定与筛选

尚维,赵申清玉,党江波,郭启高,梁国鲁,杨超,张艳,陈益银

Identification and Screening of Nicotiana tobacam-N. plumbaginifolia Heterologous Chromosome Plants Based on SSR Marker

Wei SHANG,Shen-Qing-Yu ZHAO,Jiang-Bo DANG,Qi-Gao GUO,Guo-Lu LIANG,Chao YANG,Yan ZHANG,Yi-Yin CHEN

图4 引物PT50178在云烟87、N. plumbaginifolia基因组DNA和部分回交后代中的扩增情况 M: marker; 箭头所指为N. plumbaginifolia扩增出的特异位点; 1~35为1~35号回交后代; ♂为N. plumbaginifolia; ♀为云烟87八倍体。

Fig. 4 Genomic DNA amplified by primer PT50178 in Yunyan 87, N. plumbaginifolia, and some backcross plants M: marker; Arrows indicate N. plumbaginifolia amplificatory specific loci; 1-35 indicate 1-35 BC1 plants; ♂: N. plumbaginifolia; ♀: Yunyan 87 octaploid.