拟南芥RPT2与RIP1互作调节下胚轴向光弯曲的功能鉴定

赵翔,朱自亿,王潇楠,慕世超,张骁

Functional Analysis of Hypocotyl Phototropism Modulated by RPT2-Interacting Protein RIP1 in Arabidopsis thaliana L.

Xiang ZHAO,Zi-Yi ZHU,Xiao-Nan WANG,Shi-Chao MU,Xiao ZHANG

图2 拟南芥RPT2互作蛋白酵母库筛选体系建立 A: 重组酵母双杂交载体的构建; B: 表达蛋白的毒性检测; C: 酵母库筛选诱饵蛋白自激活验证; D: 酵母库筛选结果显色分析。

Fig. 2 Screening of proteins interacted with RPT2 by yeast two hybrid A: construction of recombinant yeast two hybrid vector; B: analysis of toxicity of recombinant yeast two-hybrid vector; C: validation of autonomously activate of bait gene; D: the color analysis of vectors of Yeast library screening.