小麦热激转录因子基因TaHsfA2e特性及耐热性功能初探

张玉杰,张园园,张华宁,秦宁,李国良,郭秀林

Characterization and Regulatory Roles in Thermotolerance of Wheat Heat Shock Transcription Factor Gene TaHsfA2e

Yu-Jie ZHANG,Yuan-Yuan ZHANG,Hua-Ning ZHANG,Ning QIN,Guo-Liang LI,Xiu-Lin GUO

图4 50℃热激处理后转基因TaHsfA2e和空载体酵母细胞耐热性观察 A: 30°C下正常生长, B: 50°C热胁迫 45 min, 于30°C恢复生长 3 d; C: 30°C下正常生长测定酵母细胞OD600值; D: 50°C热胁迫 45 min后于30°C恢复生长测定酵母细胞 OD600值。数据为3个生物学重复平均值±标准差。*表示转基因酵母与对照(转空载体)差异显著(P<0.05)

Fig. 4 Thermotolerances of yeast harboring pYES2-TaHsfA2e and pYES2 after heat shock at 50°C A: culture at 30 °C; B: HS at 50 °C for 45 min, then culture at 30 °C for 3 days; C: OD600 of transformed yeast cells at 30 °C; D: OD600 of transformed yeast cells at 50 °C for 45 min and then culture at 30 °C. The error bar represents ±SD of triplicate experiments. * means significant difference between transgenic yeast cells and the controls (harboring pYES2) (P<0.05)