小麦热激转录因子基因TaHsfA2e特性及耐热性功能初探

张玉杰,张园园,张华宁,秦宁,李国良,郭秀林

Characterization and Regulatory Roles in Thermotolerance of Wheat Heat Shock Transcription Factor Gene TaHsfA2e

Yu-Jie ZHANG,Yuan-Yuan ZHANG,Hua-Ning ZHANG,Ning QIN,Guo-Liang LI,Xiu-Lin GUO

图5 3个转TaHsfA2e拟南芥株系及野生型的基础耐热性和获得耐热性鉴定 转基因系(8_1, 12_2, 14_19)和野生型种子播种于平皿培养基上, 经3 d黑暗孵育、光照5 d后分别进行热激处理。A: 正常生长的植株; B: 基础耐热性处理的植株(45℃ 40 min, 再于22℃恢复8 d); C: 获得耐热性处理植株(37℃ 1 h, 22℃下恢复2 d, 46℃ 50 min, 最后22℃恢复8 d); D: 转基因株系和野生型在正常生长(CK)、基础耐热性处理(BT)和获得耐热性处理(AT)条件下的存活率, 实验重复3次, 每重复至少15株苗。

Fig. 5 Assays of basal and acquired thermotolerance in three TaHsfA2e-transgenic Arabidopsis and its wild type (WT) The seeds of TaHsfA2e transgenic Arabidopsis lines (8_1, 12_2, and 14_19) and wild type were planted in plate, incubated for 3 d, and grew for 5 d. Then all seedlings were subjected to heat stress. A: plants under normal growth conditions; B: plants subjected to HS treatment of BT (45°C 40 min, recovered growth for 8 d at 22°C); C: plants subjected to HS treatment of AT (37°C 1 h, recovered growth for 2 d at 22°C, and 46°C 50 min, then recovered growth for 8 d at 22°C); D: survival rates of transgenic and wild type plants at normal conditions and subjected to BT and AT treatments. The represented values are the means of at least 15 individual plants of each line, and the experiment was repeated three times.